核因子κB活性在心肺脑复苏后的变化  

The role and significance of nuclear factor-kappa B activity after cardiopulmonary-cerebral resuscitation

作　　者：李欣[1] 荆小莉[1] 熊艳[1] 蔺际龚[1] 李玉杰[1] 马中富[1] 廖晓星[1] 

机构地区：[1]中山大学附属第一医院急诊科,广州510080

出　　处：《中华急诊医学杂志》2006年第1期19-22,共4页Chinese Journal of Emergency Medicine

基　　金：广州市重大科技攻关项目(2004Z3E0381)

摘　　要：目的观察心搏骤停患者经心肺脑复苏(CPCR)后核因子κB(NFκB)活性和肿瘤坏死因子α(TNFα)水平的变化及意义。方法以电泳迁移率变动分析法(EMSA)检测外周血单个核细胞的NFκB活性,放射免疫法测定血清TNFα水平。结果NFκB活性、TNFα水平和APACHEⅡ评分在复苏后多脏器功能障碍综合征(PRMODS)组和死亡组分别明显高于非PRMODS组和存活组(P<0.05);NFκB活性、TNFα水平和APACHEⅡ评分均呈正相关(P<0.05)。结论心搏骤停和CPCR过程可以使NFκB激活、TNFα表达增加,这对PRMODS的发生、病情轻重具有重要决定作用。Objective To study the change and role of nuclear factor-kappa B （NF-kB） activity and tumor necrosis factor-alpha （TNF-α） level in patients with cardiac arrest after cardiopulmonary-cerebral resuscitation （CPCR）. Methods The NF-kB activity in monocytes was measured by dectrophoretic mobility shift assays （EMSA） and the level of TNF-α in serum was measured by radioimmunoassay. Results The NF-kB activity, TNF-α level and APACHE Ⅱ score in both post-resuscitation multiple organ dysfunction syndrome （PR-MODS） group and death group were higher than those in nonPR-MODS group and survival group （ P 〈 0.05）. There was a positive correlation among NF-kB activity, TNF-α level and APACHE Ⅱ score （ P 〈 0.05）. Conclusion Cardiac arrest and the CPCR procedure can activate NF-kB and then induce high expression of TNF-α, NF-kB is therefore a good marker for the occurrence and severity of PR-MODS.

关 键 词：心肺脑复苏 复苏 多脏器功能障碍综合征 

分 类 号：R459.7[医药卫生—急诊医学]