雷帕霉素对烫伤后金黄色葡萄球菌脓毒症大鼠白细胞介素10表达的影响  

Effect of rapamycin(RPM)on interleukin-10 gene expression in rats with postburn Staphylococcus aureus sepsis

作　　者：姚胜[1] 姚咏明[1] 李红云[1] 于燕[1] 盛志勇[1] 

机构地区：[1]解放军总医院第三○四临床部烧伤研究所,北京100037

出　　处：《中华急诊医学杂志》2006年第1期34-37,共4页Chinese Journal of Emergency Medicine

基　　金：国家重点基础研究发展规划资助项目(G1999054203;2005CB522602);国家杰出青年科学资助项目(30125020);首都医学发展科研基金重点资助项目(20032023)

摘　　要：目的探讨脓毒症时应用雷帕霉素(RPM)干预对肝脏内源性白介素10(IL10)表达和急性肝损伤的影响。方法采用烫伤后金黄色葡萄球菌感染致严重脓毒症大鼠模型,50只大鼠随机分为正常对照组(n=5)、烫伤对照组(n=5)、烫伤脓毒症组(n=30)和RPM处理组(n=10)。采用逆转录多聚酶链式反应测肝组织IL10mRNA表达,并应用ELISA方法分别检测肝组织、血中IL10蛋白水平,同时观察肝功能指标的改变。结果与烫伤脓毒症组比较,RPM干预后0.5h肝组织中IL10mRNA表达和血中IL10蛋白水平明显升高(P<0.05),干预后2h则有不同程度的下降,但仍显著高于伤前水平(P<0.05或P<0.01)。同时,RPM干预组肝功能酶学指标明显改善。结论烫伤脓毒症早期应用RPM有助于保护IL10的内源性抗炎效应,从而减轻失控性炎症反应和急性肝损伤。Objective To investigate the effect of rapamycin （RPM） on hepatic interleukin-10 （IL-10） gene and acute liver injury in rats with postburn Staphylococcus aureus sepsis. Methods Fifty male Wistar rats were randomly divided into four groups： normal control group, scald control group, postburn sepsis group, and RPM treatment group. Tissue samples from liver and plasma were collected to determine IL-10 mRNA and protein expressions, and liver function parameters were also measured. Results Compared to postburn Staphylococcus aureus sepsis group, in RPM treatment group hepatic IL-10 mRNA expression and plasma IL-10 were significantly increased at 0.5 hour after RPM treatment （ P 〈 0.05）, while they were decreased at 2 hours, but the values were markedly higher than those in normal controls. Liver function parameters were significantly improved in septic animals treated with RPM. Conclusion Early treatment with RPM could regulate endogenous IL-10 expression and release, so prevent excessive inflammatory response and attenuate acute liver injury secondary to postburn Staphylococcus aureus sepsis.

关 键 词：脓毒症 雷帕霉素 白细胞介素10 急性肝损伤 

分 类 号：R644[医药卫生—外科学]