有机磷农药对乙酰胆碱酯酶活性的联合抑制作用  

Combined inhibitory effects of organophosphorous pesticides on acetylcholinesterase activity

作　　者：颜冬云[1] 蒋新[1] 余贵芬[1] 卞永荣[1] 谷成刚[1] 杨兴伦[1] 

机构地区：[1]土壤与农业可持续发展国家重点实验室,中国科学院南京土壤研究所,江苏南京210008

出　　处：《农药》2006年第1期31-34,共4页Agrochemicals

基　　金：国家重点基础研究发展规划项目(2002CB410805);中国科学院知识创新工程领域前沿项目(ISSASIP0202)

摘　　要：为研究二元有机磷农药对乙酰胆碱酯酶活性的影响,分别测定了21种有机磷农药对乙酰胆碱酯酶的半抑制浓度IC50,在此基础上用等毒法测定了二元有机磷农药对乙酰胆碱酯酶的联合抑制作用。结果表明有机磷农药对乙酰胆碱酯酶的联合抑制作用主要表现为协同作用与相加作用,拮抗作用较少发生。量子化学参数分析表明具有相似电子效应的二元混合有机磷农药对乙酰胆碱酯酶的抑制主要表现为协同作用,而电子效应相差较大的二元混合有机磷农药对乙酰胆碱酯酶的抑制主要表现为拮抗作用。The concentrations causing 50% inhibition （IC50） of acetylcholinesterase activity by 21 organophosphorus pesticides were determined in order to study the effects of organophosphorus pesticide combinations on acetylcholinesterase. On this basis, the joint inhibitory effects of organophosphorus pesticide combinations on acetylcholinesterase activity were examined using a toxic equivalency method. Results showed that joint inhibitory effects of organophosphorus pesticides on acetylcholinesterase primarily were synergistic and additive, with only rare antagonistic effects. Quantum chemical analysis further suggested that binary organophosphorus pesticide mixtures with similar electronic effects showed synergistic inhibition of acetylcholinesterase. However, mixtures with dissimilar electronic effects showed antagonistic inhibitory effects.

关 键 词：有机磷农药 乙酰胆碱酯酶 量子化学参数 联合抑制作用 

分 类 号：TQ450[化学工程—农药化工]