丙泊酚对大鼠海马脑片CA1区长时程增强的影响  

Effect of propofol on long term potentiation in CA1 region of rat hippocampal slices

作　　者：尚游[1] 姚尚龙[1] 江山[2] 曾因明[2] 刘红亮[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院麻醉学教研室,武汉市430022 [2]徐州医学院江苏省麻醉学重点实验室

出　　处：《临床麻醉学杂志》2006年第1期49-51,共3页Journal of Clinical Anesthesiology

基　　金：江苏省教育厅开放课题(KJS03084)

摘　　要：目的观察不同浓度丙泊酚对离体大鼠海马脑片CA1区长时程增强(LTP)的影响。方法30张海马脑片分为五组,Ⅰ、Ⅱ和Ⅲ组分别应用浓度为30、10和3μmolo/L的丙泊酚,Ⅳ组用脂肪乳,Ⅴ组不用药物作为对照。利用细胞外记录方式,以海马脑片CA1区群峰电位(PS)为观察指标,首先观察丙泊酚对CA1区基础传递的影响,待基线稳定后,记录高频刺激(HFS)后海马脑片CA1区PS的变化情况。结果Ⅰ、Ⅱ、Ⅲ组应用丙泊酚后PS降低,在持续给药后30min恢复至基线。实施HFS后,Ⅲ、Ⅳ和Ⅴ组的PS较HFS前显著升高(P<0.05,P<0.01);而Ⅰ、Ⅱ组PS与HFS前相比差异无显著意义(P>0.05)。HFS后,Ⅰ组PS显著低于Ⅱ、Ⅲ、Ⅳ和Ⅴ组(P<0.01),Ⅱ组PS也低于Ⅲ、Ⅳ和Ⅴ组(P<0.05)。结论丙泊酚可以抑制大鼠离体海马脑片CA1区LTP的形成。Objective To observe the effect of propofol on long term potentiation （LTP） in CA1 region of rat hippocampal slices. Methods Thirty hippoeampal slices were divided into 5 groups of 30μmok/L （groupⅠ）, 10μmol/L （groupⅡ）, 3μmol/L （groupⅢ） propofol, intralipid （groupⅣ）and control （groupⅤ ）. Population spikes （PS） in CA1 region were evoked by extracellular microelectrode recording technique. First, effect of propofol on baseline of PS was investigated. LTP was induced by high frequency stimulation （HFS） when baseline was stable. Results Amplitude of PS decreased after propofol was applied. However, amplitude returned to baseline 30 min later. Amplitude of PS after HFS in group Ⅲ , group Ⅳand groupⅤ increased significantly compared with the baseline （P〈0. 05, P〈0.01）. However, amplitude of PS after HFS in group Ⅰ and group Ⅱ were similar to the baseline （P〈0.05）. The amplitude of PS in group Ⅰwas lower than that in group Ⅱ ,Ⅲ, Ⅳ, Ⅴafter HFS （P〈0.01）. The amplitude of PS in group Ⅱwas also lower compared with that of group Ⅲ, Ⅳ, Ⅴ（P〈0.05）. Conclusion Propofol may inhibit the induction of LTP in hipp ocampal slices of rats in vitro.

关 键 词：丙泊酚 海马脑片 长时程增强 

分 类 号：R614[医药卫生—麻醉学]