奶牛隐孢子虫的检测技术  

DETECTIVE TECHNOLOGIES OF CRYPTOSPORIDIUM IN DAIRY CATTLE

作　　者：黄燕[1] 徐梅倩[1] 何国声[1] 曹杰[1] 朱顺海[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所,上海200232

出　　处：《中国兽医寄生虫病》2006年第1期32-38,共7页Chinese Journal of Veterinary Parasitology

基　　金：国家科技部基础工作专项;畜禽重要寄生虫虫种资源的收集与保存(编号:2002DEB10050);上海科技兴农重点攻关项目(编号:2004-12-3)

摘　　要：隐孢子虫病是一种重要的人畜共患原虫病。在家畜中以对反刍动物—奶牛的危害最大。当前,奶牛隐孢子虫主要还是以粪便检测为主,如改良抗酸染色法、金胺—酚改良抗酸复染法等。现在还有应用日趋广泛的免疫学检测方法,如ELISA、IFA、M cAb等;分子生物学检测方法,如常规PCR、巢式PCR等。本文对上述各种检测方法作一综述,以供广大寄生虫病学和兽医工作者参考。Cryptosporidiosis is a important protozonic zoonosis. In livestock, it harms ruminant-dairy catties badly. Now, detecting Cryptosporidium oocysts in dairy cattle is mainly fccai-testing, including classical methods-Modified acid-fast staining, Auramine-phenol staining, immunological methods-ELISA, IFA, McAb, and molecular biological mothods-PCR, Nested PCR and so on. So this article with sum up different kinds of means for detecting. All of these will be supplied as references for personnel of researching parasites.

关 键 词：隐孢子虫病 检测 粪便 奶牛 

分 类 号：S858.232.723[农业科学—临床兽医学]