冬虫夏草提取液对小鼠病毒性心肌炎心肌脂质过氧化作用的影响  

Effect of Extract of Cordyceps Sinensis on Lipid Peroxidation of Mice with Viral Myocarditis

作　　者：李锋[1] 高兴玉[2] 饶邦复[3] 刘利[2] 崔连群[1] 

机构地区：[1]山东大学山东省立医院,山东济南250021 [2]川北医学院附属医院,四川南充637007 [3]重庆医科大学第一附属医院,重庆400016

出　　处：《南京中医药大学学报》2006年第1期21-23,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(39870955);四川省教育厅重点课题(01LA32)

摘　　要：目的了解早期应用冬虫夏草提取液(CSAE)治疗对小鼠病毒性心肌炎(VMC)心肌超氧化物歧化酶(SOD)、丙二醛(MDA)及诱导型一氧化氮合酶(iNOS)的影响及意义。方法100只雄性BALB/c鼠随机分为正常对照组、感染组和CSAE治疗组。感染组、CSAE治疗组小鼠腹腔接种柯萨奇B3病毒,并于接种病毒后d 7、d 14分批处死。测定心肌SOD、MDA含量及iNOS表达,光镜下心肌病变半定量评分。结果与感染组比较,CSAE治疗组接种病毒后d 7及d 14心肌SOD活性i、NOS表达均显著增高(P<0.01),而MDA含量降低(P<0.01),心肌炎症损伤减轻。结论CSAE通过增加心肌抗氧化酶活性及诱导iNOS表达减轻VMC小鼠心肌脂质过氧化损伤。OBJECTIVE To understand the effect and significance of extract of Cordyceps Sinensis on mice with viral myocarditis. METHOD 100 male BALB/c mice were randomly divided into a control group, an infection group and a group treated with extract of Cordyceps Sinensis. The mice in the infection group and the group treated with extract of Cordyceps Sinensis were inoculated with Cox- sackie B3 virus and were then killed seven and fourteen days respectively after the inoculation. Myocardium SOD, MDA and iNOS expressions were determined and semiquantitative scoring of the pathological changes of the heart was carried out under the optical microscope. RESULT Compared with the infection group, SOD activity and iNOS expressions in the group treated with extract of Cordyceps Sinensis increased markedly （ P 〈 0.01 ）, MDA level decreased （ P 〈 0.01 ） and myocarditis was relieved. CONCLUSION Extract of Cordyceps Sinensis can alleviate lipid perexidation of mice myocardium by increasing active antioxidase and inducing iNOS expression.

关 键 词：病毒性心肌炎 冬虫夏草提取液 超氧化物歧化酶 丙二醛 诱导型一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]