用4对引物聚合酶链式反应检测鼠疫耶尔森氏菌的实验研究  

Study on detection of Yersinia pestis by polymerase chain reaction using 4 pairs of primer

作　　者：杜国义[1] 史献明[1] 牟振云[2] 李玉贵[1] 白万翔[1] 刘合智[1] 白雪微[1] 陈永明[1] 

机构地区：[1]河北省鼠疫防治所,张家口075000 [2]河北医科大学

出　　处：《中国地方病防治》2006年第1期10-12,共3页Chinese Journal of Control of Endemic Diseases

摘　　要：目的用4对引物聚合酶链式反应(以下简称4-Prim er-PCR)来检验鼠疫耶尔森氏菌,对于探索鼠疫的快速诊断方法将起到推动作用。方法选择不同疫源地的40株鼠疫耶尔森氏菌;目的基因选择于与鼠疫耶尔森氏菌毒力因子有关的FI抗原质粒基因cafI鼠疫杆菌素Ⅰ基因、与色素沉着有关的质粒基因hm s以及染色体基因inv。结果该法所有的鼠疫耶尔森氏菌全部出现4对引物的预期扩增带;该试验在4 h内完成;结论四对引物鼠疫PCR法具有快速、特异、敏感等优点,可以用于鼠疫的快速诊断和流行病学调查。Objective A 4 - primer - PCR method for decetection of Yersina pestis is used to explore a quickly diagnosis of plague. Methods 40 Yersini pestises come from diffirent foci. Four pairs of olignuelotide primer are designed from each gene of three kinds of virlent plamids and a ehronlsomed DNA; 60-plasmid-located gene earl encoding Y. pestis specil virlent fraction, a Y. pestis speeil of yop encoding Y. pestis specil 42 - Md virlent plamids, a plasmiden activator gene （pla） encoding Y. pestis specil 7 - Md, and an invasion protein gene （inv） encoding on chomosomed DNA. Results Expected cares are found in 4 - Prmer - PCR. All the tests are completed in 4 hours. Conclusions 4 - pimer - PER is simple and quick and will be useful for diagnosis of plague in eases of emergency and for surveillance of epidenlics.

关 键 词：4对引物 聚合酶链式反应 鼠疫耶尔森氏菌 

分 类 号：R516.8[医药卫生—内科学]