褐藻叉开网翼藻化学成分研究  

Studies on chemical constituents of the brown alga Dictyopteris divaricata

作　　者：宋福行[1] 范晓[1] 徐秀丽[1] 王素娟[2] 李帅[2] 杨永春[2] 石建功[2] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国医学科学院中国协和医科大学药物研究所,北京100050

出　　处：《中国中药杂志》2006年第2期125-128,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学重点基金资助项目(20432030);国家高技术发展计划(863计划)专项(2001AA620403)

摘　　要：目的:对网地藻科褐藻叉开网翼藻进行化学成分研究,并对分离得到的化合物进行细胞毒活性实验。方法:利用正相硅胶柱色谱、Sephadex LH-20柱色谱、反相HPLC及重结晶等手段进行分离纯化;借助IR,MS及NMR鉴定结构;通过改良的MTT法对单体化合物进行细胞毒活性实验。结果:从叉开网翼藻中分离得到8个化合物,分别鉴定为(-)-torreyol(Ⅰ),4β,5-αdihydroxycubenol(Ⅱ),3-farnesyl--phydroxybenzioc acid(Ⅲ),chromazonarol(Ⅳ),岩藻甾醇(Ⅴ),苯乙酰胺(Ⅵ),4-羟基苯甲酸(Ⅶ)和正十六酸(Ⅷ)。结论:化合物Ⅱ和Ⅳ系首次从该海藻中发现;其余化合物均为首次从网地藻科海藻中分离得到。细胞毒活性试验结果显示所有化合物均无明显活性(IC50>10μgmL-1)。Objective： To investigate the chemical constituents of the brown alga D. divaricata, and to test cytotoxicities of the purified compounds. Method： Compounds were isolated by normal phase silica gel, Sephadex LH-20 chromatography and reverse phase HPLC techniques. Their structures were elucidated by spectroscopic methods including IR, MS and NMR. Cytotoxicities were tested by MTT method. Result： Eight compounds were isolated from ethanolic extract of the brown alga D. divaricata and their structures were identified as （ - ）-torreyol （ Ⅰ）, 4β, 5α-dihydroxycubenol （ Ⅱ ）, 3-farnesyl-p-hydroxybenzioc acid （ Ⅲ）, chromazonarol （Ⅳ）, fucosterol （ Ⅴ ）, phenyl acetylamine （ Ⅵ ）, 4-hydroxybenzoic acid （ Ⅶ） and n-hexadecanoic acid （Ⅷ）. Conclusion： Compound Ⅱ and Ⅳ were obtained from this alga for the in-st time. The others were isolated from the Dictyotaceae algae for the first time. All compounds were inactive （IC50 〉 10 μg·mL^- 1 ） a- gainst human tumor cell lines KB, Bel-7402, PC-3M,Ketr 3 and MCF-7.

关 键 词：褐藻 叉开网翼藻 化学成分 

分 类 号：R284[医药卫生—中药学]