茅莓总皂苷对大鼠局灶性脑缺血的保护作用  

The preotective effects of total glycosides Rubus parviflolius on cerebral ischemica in rat

作　　者：王继生[1] 邱宗荫[1] 夏永鹏[1] 李惠芝[1] 任凌燕[1] 张莉[1] 

机构地区：[1]重庆医科大学药学系,重庆400016

出　　处：《中国中药杂志》2006年第2期138-141,共4页China Journal of Chinese Materia Medica

基　　金：重庆市重大科技专项资助课题(渝科发计字(2004)27号)

摘　　要：目的:研究茅莓总皂苷(TGRP)对局灶性脑缺血的保护作用。方法:采用大脑中动脉阻塞(MACO)法制备大鼠局灶性脑缺血模型;脑梗死区重量用TTC染色法测定,超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)及细胞凋亡均采用测试药盒测定。结果:TGRP 10,20 mg.kg-1可改善大鼠异常神经症状,使MACO大鼠的SOD,GSH-Px活力提高,MDA生成减少,减少脑皮层细胞凋亡数。结论:TGRP对脑梗死有保护作用,其作用机制可能与抗自由基、抑制细胞凋亡有关。Objective： To observe the protective effects of total glycosides Rubus parviflolius （TGRP） on local cerebral ischemic. Method： The local cerebral ischemia in rat was made by middle cerebral artery occlusion（MACO）. The infraction weight was determined by TTC stain. SOD, MDA, GSH and apoptotis were determined with different method respectively. Result： TGRP 20, 10 mg·kg^-1ig markedly improved the abnormal nervous symptoms, incredsed the SOD, GSH activity and reduced contentes of MDA in brain of MACO rat, TGRP 20 mg·kg^- 1ig significantly decreased the numbers d apoptotic cells in ischemic cortex, Condusion： TGRP has protective effects against cerebral infrction, and its mechanism may be related to anti-apoptotis and free radical.

关 键 词：茅莓总皂苷 大脑中动脉阻塞 超氧化物歧化酶 丙二醛 谷胱甘肽过氧化物酶 凋亡 

分 类 号：R285.5[医药卫生—中药学]