植物细胞多倍体实验材料培养方法的改进  

Improvement of Inducing Plant Polyoloid in Experiment

作　　者：彭晓云[1] 张明[1] 许东祝[1] 

机构地区：[1]韶关学院英东生物工程学院,广东韶关512005

出　　处：《韶关学院学报》2005年第12期82-83,88,共3页Journal of Shaoguan University

摘　　要：用4种浓度(0.01%,0.03%,0.05%,0.10%)的秋水仙素,分四个不同的处理时间(1 d,2 d,3 d,4 d),对3种材料(洋葱,蚕豆,大蒜,带皮和去皮)进行染色体加倍处理.结果表明:在25℃的培养箱中,用0.05%秋水仙素处理3 d的去皮大蒜,诱导多倍体率最高,为445%,足以满足学生实验需要.The chromosomes of onion, horsebean and garlic with hull and without it were doubled using 0.01% ,0.03 %, 0.05%, 0.1% colchicines treatment in different treatment time （1 - 4 days）. The results were as follows： most effective inducing way was garlic without hull to treat with 0.05 % colchicines solution at 25 ℃ for 3 days, and polyploid inductivity is 445 %, which can satisfy need of student experiments.

关 键 词：秋水仙素 多倍体 培养方法 诱导 

分 类 号：Q343.244[生物学—遗传学]