Direct Observation of Histone-Induced DNA Shortening  

作　　者：冉诗勇 王晓玲 付文博 赖振华 王渭池 刘晓晴 麦振洪 李明 

机构地区：[1]Belting National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080 [2]Life Science College, Capital Normal University, Beijjng 100037

出　　处：《Chinese Physics Letters》2006年第2期504-507,共4页中国物理快报（英文版）

基　　金：Supported by the National Natural Science Foundation of China under Grant No 10334100. The authors acknowledge the help of Professor .J. Yan of the National University of Singapore in setting up the instrument.

摘　　要：We construct a system of magnetic tweezers and apply it to study the interaction between histones and DNA. The condensation of DNA by purified histones at low ionic strengths is directly monitored by recording the length of the DNA as a function of elapsed time. It is found that DNA condensates in a dynamic manner. The binding of hist, ones to DNA is energetically favoured, but the ten,sion applied on DNA tends to unravel the DNA-histone complex, The competition between the two processes determiners the rate of the DNA condensation.We construct a system of magnetic tweezers and apply it to study the interaction between histones and DNA. The condensation of DNA by purified histones at low ionic strengths is directly monitored by recording the length of the DNA as a function of elapsed time. It is found that DNA condensates in a dynamic manner. The binding of hist, ones to DNA is energetically favoured, but the ten,sion applied on DNA tends to unravel the DNA-histone complex, The competition between the two processes determiners the rate of the DNA condensation.

关 键 词：SINGLE CHROMATIN FIBERS SUPERCOILED DNA RNA-POLYMERASE STRANDED-DNA MOLECULES ELASTICITY FORCE NUCLEOSOMES RESOLUTION TWEEZERS 

分 类 号：Q523[生物学—生物化学] Q6