电脉冲刺激肾旁穴对兔丘脑雌激素受体蛋白表达的影响  

Effects of Shenpang Acupoint-stimulation on Estrogen Receptors Immunoreactive Neurons in Thalamus in Rabbits

作　　者：陈正礼[1] 罗启慧[2] 范光丽[2] 方静[1] 袁朝富[1] 潘康成[1] 牛建荣[3] 杨国林[3] 

机构地区：[1]四川农业大学动物科技学院,雅安625014 [2]西北农林科技大学动物科技学院,杨凌712100 [3]中国农业科学院兰州畜牧与兽药研究所,兰州730050

出　　处：《畜牧兽医学报》2006年第1期71-75,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目(3967055039870551)

摘　　要：采用电脉冲刺激穴位法及免疫组织化学SP法,研究了电脉冲刺激肾旁穴对丘脑内雌激素受体免疫反应阳性产物的影响。结果发现雌激素受体免疫反应阳性产物在丘脑内广泛分布,可见电针组丘脑室旁核、丘脑腹外侧核、丘脑腹内侧核、丘脑腹主核、丘脑中央中核、丘脑网状核、丘脑室周核等核区有大量阳性产物,且多分布于神经元的胞浆、胞核和突起中、有少部分分布于胞膜上,染色较深,突起明显,细胞形态多样,轮廓清晰;而对照组相应神经核团中阳性细胞数量较少且淡染,突起也不明显,形态单一或轮廓不清。两组阳性产物的染色强度和细胞数目均差异显著。以上结果表明电脉冲刺激肾旁穴可使家兔上述核团内雌激素受体的表达增强。For investigating the effects of Shenpang acupoint-stimulation on reproductive endocrine, the changes of estrogen receptors immunoreactive （ER-IR） neurons after Shenpang acupoint-stimulation were studied by using the technique of immunohistochemistry SP. The ER IR positive productions were detected in most nuclei of thalamus. In acupuncture group, a great number of ER IR positive productions, with clear dendrite, existed in the nucleus paraventricularis thalami, nucleus ventralis lateralis thalami, nucleus ventralis medialis thalami, nucleus vent- ralis principalis thalami, nucleus centromedianus thalami, nucleus reticularis thalami, nucleus periventrieularis thalami, and were stained strongly. In addition, the ER-IR positive production mainly located in plasma, nucleus and neurite, also existed in the cytoplasmic membrane. In contrast, a few of neurons existed in the above nuclei in control group, and the neurons were stained weakly. The expression of estrogen receptors in the above nuclei increased after the Shenpang acupoint-stimulation.

关 键 词：兔 电针 肾旁穴 雌激素受体 丘脑 

分 类 号：S852.16[农业科学—基础兽医学]