L－半胱氨酸的停流流动注射动力学快速测定法  

Rapid Kinetic Determination of L-Cysteine by Stopped-flow Analysis

作　　者：曾飒[1] 谭辉玲[1] 

机构地区：[1]中山医科大学化学教研室,重庆大学应用化学系

出　　处：《分析测试学报》1996年第3期78-81,共4页Journal of Instrumental Analysis

摘　　要：利用Ｌ－半胱氨酸可还原Ｆｅ（３＋），使之生成Ｆｅ（２＋）－邻菲　啉有色络合物的反应，建立了Ｌ－半胱氨酸的停流流动注射动力学分光光度测定的新方法。该法简便、灵敏、快速、准确、选择性高，检测限为１６ｍｇ／Ｌ，分析频率达３０样／ｈ。取Ｌ－半胱氨酸浓度为１６０ｍｇ／Ｌ时，测量的相对标准偏差为０．５５％（ｎ＝６）。实际样品加标回收率达９８．７％。A rapid kinetic stopped-flow method for L-cysteine determination is described, which is based on the colored complex formation between L-cysteine, iron(Ⅲ) and 1, 10-phenanthroline with a maximum absorbance at 508 nm. The injected sample volume is 157μL. The linear range of the calibration curve is 16￣ 280 mg/L. The relative standard deviation is 0. 55% (n= 6) at a L-cysteine concentration of 120 mg/L. The sampling frequency is 30h￣(-1). The method is simple, rapid, sensitive, accurate and highly selective. It has been applied to the determination of L-cysteine in strawberry juice, and has a recovery rate of 98. 7%.

关 键 词：L-半胱氨酸 流动注射分析 分光光度法 动力学 

分 类 号：O623.736[理学—有机化学] O657.32[理学—化学]