葡萄球菌溶酶对h-VRSA溶菌作用观察  

The Study of the Bactericidal Efficacy of Lysostaphin to Heterogeneous Vancomycin-resistant Staphylococcus Aureus

作　　者：王敬华[1] 马筱玲[2] 刘励军[1] 姚辉华[1] 

机构地区：[1]苏州大学附属第二医院检验科,江苏苏州215004 [2]安徽省立医院检验科,安徽合肥230001

出　　处：《现代检验医学杂志》2006年第1期1-4,共4页Journal of Modern Laboratory Medicine

基　　金：安徽省"十五"科技攻关基金资助(01013039)

摘　　要：目的从金黄色葡萄球菌临床分离菌株中检测异质性万古霉素耐药金黄色葡萄球菌(heterogeneous vancom yc in-res istan t staphy lococcus aureus,h-VRSA),并考察葡萄球菌溶酶对h-VRSA体外杀菌作用。方法采用E-test法从金黄色葡萄球菌临床分离菌株中筛选h-VRSA,采用棋盘稀释法考察葡萄球菌溶酶单独或联合万古霉素对h-VRSA体外杀菌作用。结果从30株金黄色葡萄球菌临床分离菌株中检测出2株h-VRSA(6.7%),葡萄球菌溶酶联合万古霉素对h-VRSA溶菌作用的部分抑菌浓度指数:F IC<0.5。结论金黄色葡萄球菌临床分离菌株中有h-VRSA存在,其对万古霉素表现出的异质性耐药不容忽视;葡萄球菌溶酶和万古霉素之间有良好的协同作用,其临床应用价值值得进一步探讨。Objective To detect heterogeneous vancomycin-resistant staphylococcus aureus （h-VRSA） from the clinical isolates of staphylococcus aureus,and study the bactericidal efficacy of lysostaphin to h-VRSA. Methods Method of E-test was used to detect h-VRSA from 30 isolates of clinical isolated staphylococcus aureus,the bactericidal efficacy of lysostaphin solely or combine with vancomycin to h-VRSA were studied. Results 2 subclones of h-VRSA were isolated（6.7%）,the fractional inhibitory concentration：FIC〈0. 5. Conclusion There are h-VRSAs （6.7 %）in clinical isolates of staphylococcus aureus,and the heterogeneous vancomycin-resistant of staphylococcus aureus,shouldn＇t be overlooked;lysostaphin combine with vancomyein may bring a coordinated bactericidal efficacy to h-VRSA,so the clinical application of lysostaphin combine with vancomycin to treat the infection of h-VRSA should be studied further more.

关 键 词：异质性万古霉素耐药金黄色葡萄球菌 E—test 葡萄球菌溶酶 

分 类 号：R446.5[医药卫生—诊断学]