MDQ型毛细管电泳仪分离血清脂蛋白方法学建立与评价  

Analysis and Evaluate of Serum Lipoproteins by MDQ Model Capillary Electrophoresis Apparatus

作　　者：王跃国[1] 王惠民[1] 张芹[1] 杨曙梅[1] 黄一红[1] 王忠慧[1] 

机构地区：[1]南通大学附属医院检验医学中心,江苏南通226001

出　　处：《现代检验医学杂志》2006年第1期15-18,共4页Journal of Modern Laboratory Medicine

基　　金：江苏省卫生厅重大项目(H200216);南通市社会发展基金资助课题(2004-S40031)

摘　　要：目的建立毛细管区带电泳分离血清脂蛋白的方法。方法用75μm(i.d.)×60 cm(L)未涂层熔融硅毛细管,含0.001 g/d l B rij35 pH 10.0的硼砂溶液作电泳缓冲液,分离经NBD-酰基鞘氨醇预染的血清脂蛋白,激光诱导荧光(L IF)检测。结果10 m in内可将脂蛋白分成3条区带。结论毛细管区带电泳为血清脂蛋白分析提供了一种新的分析方法。该法简单、快速、重复性好。Objective To establish a method of serum lipoproteins by capillary zone electrophoresis. Methods The separation of pre-stained lipoproteins by NBD-ceramide was demonstrated in fused-silica capillaries with laser-induced fluorescence detector. The separation was accomplished using horax buffer （pH 10. 0）with 0.001 g/dl Brij35 and the capillary column with 75μm（i. d. ）and 60cm of total length（50cm to detector）. Results Three patterns of lipoproteins eluted within 10 min. Conclusion It was a new assay for serum lipoproteins by capillary zone electrophoresis. The method was simple, rapid and reproducible for serum lipoproteins.

关 键 词：毛细管区带电泳 血清脂蛋白 NBD-酰基鞘氨醇 Brij35 

分 类 号：Q503[生物学—生物化学] R446.112[医药卫生—诊断学]