电化学发光淬灭法测定脑白金胶囊中的褪黑素  

Determination of melatonin in capsules by quenching electrochemiluminescence analysis

作　　者：储海虹[1] 许海燕[2] 吴莹[1] 胡延维[3] 屠一锋[1] 

机构地区：[1]苏州大学化学化工学院,苏州215006 [2]南通师范学校,南通226006 [3]苏州大学医学院药学系,苏州215007

出　　处：《分析试验室》2006年第1期17-19,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金资助项目(20275025)

摘　　要：在硼酸缓冲溶液(pH 6.7)介质中,H2O2对鲁米诺的电化学发光有敏化作用,而褪黑素(MLT)因具有对自由基的湮灭作用而对此敏化的电化学发光具有淬灭作用,该发光体系的ECL光强与MLT的浓度成线性关系,检测灵敏度极高,检测下限可达到1×10-14mol/L,用于脑白金胶囊中MLT的测定得到满意结果,为MLT的测定提供了一种灵敏、可靠的测定方法。In the borate buffer solution of pH 6.7, the melatonin（MLT） quenched the H2O2 enhancing electrochemiluminescense（ECL） of luminol because of the annihilation to the oxygen free radicals. It may be sensitively determined by this method. The ECL luminous intensity linearly decreases with increase of the concentration of MLT, the detection limit is 1×10^-14mol/L, and the capsule samples have been determined with satisfactory results. It should be a sensitive and reliable method for the determination of melatonin.

关 键 词：褪黑素 电化学发光 淬灭 鲁米诺 脑白金胶囊 

分 类 号：O657.1[理学—分析化学]