基于Ag/AgCl丝双涂膜司帕沙星选择电极的研制与应用  

Preparation and application of a doubly coated sparfloxacin membrane selective electrode based on Ag/AgCl wire

作　　者：黄超伦[1] 刘静[1] 栗瑞芬[1] 修荣[1] 

机构地区：[1]河北医科大学药学院,石家庄050017

出　　处：《分析试验室》2006年第1期95-98,共4页Chinese Journal of Analysis Laboratory

基　　金：河北省科技厅攻关项目(99276422D)

摘　　要：以Ag／AgCl丝为基体依次外涂含0．1mol／L KCl的琼脂凝胶膜及含四苯硼-司帕沙星缔合物的PVC膜，制备了一种双涂膜司帕沙星选择电极。采用正交设计法，研究了离子缔舍物的种类、活性物在膜中的浓度及增塑剂三因素对电极的影响。电极的线性范围为1．0×10^-5～1．0×10^-3mol／L，检出限为2．4×10^-6mol／L，斜率为28．8mV／decade（16℃），可直接用于司帕沙星片剂的含量测定，回收率为99．6％～101．1％，结果与文献法一致。A doubly coated sparfloxacin membrane selective electrode was prepared. An Ag/AgCl wire was eoated by a layer of agar gel membrane containing 0.1 mol/L KCl and a layer of PVC membrane containing sparfloxacin-tetraphenylboron ion-pair eomplex on its suffaee successively. The influences of various ion-pair complexes, concentrations of activators in membrane phase and plastieizers on the properties of electrode were studied by orthogonal design. The linear response range of the electrode is 1.0×10^-5～1.0×10^-3mol/L. The deteetion limit is 2.4 ×10^-6 mol/L. The Nernst slope is 28.8 mV/deeade（16℃）. The electrode can be used for potentiometrle determination of sparfloxaein tablets direetly. The recovery is 99.6% - 101 . 1% . The results agree with UV method.

关 键 词：司帕沙星 双涂膜 离子选择电极 

分 类 号：O657.1[理学—分析化学]