中温和高温厌氧生物产氢反应器连续运行的研究  

Continuous Bio-Hydrogen Production by Mesophilic and Thermophilic Cultures

作　　者：张薇[1] 左剑恶[1] 崔龙涛[1] 邢薇[1] 杨洋[1] 

机构地区：[1]清华大学环境科学与工程系,北京100084

出　　处：《环境科学》2006年第1期63-68,共6页Environmental Science

基　　金：国家高技术研究发展计划(863)项目(2002AA601190)

摘　　要：采用2个厌氧生物产氢反应器分别在中温（37℃）和高温（55℃）下连续运行．以河底沉积物接种，葡萄糖为基质。在CSTR中成功实现了连续中温厌氧产氢，最高产氢量达8．6L／（L·d），基质产氢摩尔比（H2／葡萄糖）为1．98．以厌氧产甲烷颗粒污泥接种，蔗糖为基质。在UASB反应器中成功实现了连续高温厌氧产氢过程，最高产氢量达6．8L／（L·d）。基质产氢摩尔比（H2／蔗糖）为3．6．在高温UASB反应器中培养获得了灰白色的产氢颗粒污泥，平均粒径为0．8～1．2mm，沉速为30～40m／h，电镜观察发现其表层生长大量杆状细菌．对2种产氢污泥的总DNA进行提取和纯化，通过PCR扩增和DGGE分析，发现高温和中温厌氧产氢污泥中的大部分真细菌种类相同，但各自的优势菌种明显不同．Anaerobic biological hydrogen productions were achieved successfully in two lab-scale anaerobic hydrogen production reactors under mesophilic （37℃） and thermophilic （55℃） conditions, respectively. The mesophilic reactor, a CSTR, was operated over 4 months by seeding with river sediments and feeding with glucose solution, in which the highest hydrogen production rate was 8.6 L/（L·d） and the substrate hydrogen production molar ratio （H2/glucose） was 1.98. After seeded with anaerobic methanogenic granules, a UASB reactor was thermophilically operated by feeding with sucrose solution and during its steady operation period, the hydrogen production rate was 6.8L/（L· d） and the substrate hydrogen production molar ratio （H2/sucrose） was 3.6. Within the produced gas, the H2 percentages were about 43 % and others were CO2, no methane could be detected. Thermophilic hydrogenproducing granules were successfully cultivated in the UASB reactor, which were grey-white in color, the diameters were about 0.8- 1.2mm, and typical settling velocities were about 30-40m/h. Through SEM a great number of bacilli could be found on the surface of the granules which made the surface rough. Total DNA of these two hydrogen production sludges were extracted and purified, and the PCR and DGGE process were conducted, the results indicate that most of the eubacteria in two sludges are the same, but the dominant species are obviously different with each other.

关 键 词：中温 高温 厌氧生物产氢 产氢颗粒污泥 PCR DGGE 

分 类 号：X382[环境科学与工程—环境工程]