应用间接免疫荧光试验检测鸡贫血病病毒的研究  

Detection of Chicken Anemia Virus with an Indirect Fluorescent Antibody Test

作　　者：周方红[1] 崔现兰 幸桂香[1] 佟有恩[1] 曲立新[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所

出　　处：《中国畜禽传染病》1996年第5期21-24,共4页

摘　　要：用鸡贫血病病毒(CAV)MSB_1-TK_(5803)毒株接种4周龄SPF鸡,制备CAV高免血清,建立并应用间接免疫荧光试验跟踪检测了MSB_1-TK_(5803)标准毒株人工感染2批50只1日龄SPF鸡的胸腺、肝、脾、法氏囊、骨髓。结果表明,雏鸡接种第3天在胸腺、脾、骨髓中可见到特异性荧光,第5～26天在被检组织中均可见到特异性荧光。该试验与IBDV、HVT、MDV、LLV染细胞或组织涂片均无交叉反应。 用该法对天津、广州、沈阳、哈尔滨的鸡群进行了病原检查,其阳性率分别为35％、34.8％、26.6％、27％。An indirect fluorecsent antibody (IFA)test wns established with the hyperimmune serum to chicken anemia virus (CAV) MSB1-TK5803 strain raised in 4-Weck-SPF Chickens. Two batches of 50 SPF chickens were infected with MSB1-TK5803 strain,and the TFA test was applied to detct CAV antigens in the thymus,liver,spleen,bursa,and bone marrow of the infected chickens. Results showed that spacific fluorescence was detected in the thymns, spleen,bone marrow of chickens 3 days post exposure (PE) ,and spacific fluorescence was also detected in the tested samples of chickens 5 ~26 days PE. There was no cross reaction with IBDV, HVT, MDV, LLV, virus infected cell or tissure smears in the IFAtests. Field tests with the TFA indicated that CAV positive rates in Tianjin, Guangzhou, Shenyang, Harbin were 35%、34. 8%、26. 2% 、27% respectively.

关 键 词：鸡病 贫血病 病毒 免疫荧光试验 检测 

分 类 号：S858.316[农业科学—临床兽医学] S854.43[农业科学—兽医学]