海岛棉胚性细胞团的原生质体游离和培养  

Isolation and Culture of Protoplasts Form Embryogenic Cells Suspension Culture of Cotton (Gossypium Barbodense.)

作　　者：魏良民[1] 

机构地区：[1]新疆农科院经济作物研究所

出　　处：《新疆农业大学学报》1996年第1期68-72,共5页Journal of Xinjiang Agricultural University

摘　　要：以海岛棉无菌苗下胚轴为外植体诱导产生愈伤组织，挑选胚性愈伤组织建立悬浮培养胚性细胞系。用含有纤维素酶、半纤维素酶和离析酶的混合酶液从新海３号、新海７号和２８２这３个品种的胚性细胞悬浮培养物中游离出原生质体。原生质体用液体浅层法培养仅见再生细胞的１次分裂；用含有低融点琼脂糖的ＫＭ８Ｐ培养基进行琼脂糖珠悬浮法培养。Protoplasts were isolated from the hypocotyle-derived embryogenic suspension cultures of Gossypium barbadense cultivars Xinhai3、 Xinhai7、282 and Giza70.The protoplasts were released enzymatically within 12 to 14 hours in dark.The enzymes consiste of cellulase、macerozyme and hemicellulase. Protoplasts were cultured in a modified KM8P medium containing agarose of low melting point and supplented with 0.05-0.01 mg/L 2.4-D and 0.1-0.5 mg/L kinetin in droplets or thin lagers in dark.The regenerated cells started to divide after 4-5 days.Microcolonies of several cells were formed after two weeks and large colonies of more than twenty cells were formed after three weeks.

关 键 词：棉花 海岛棉 胚性细胞悬浮系 原生质体 

分 类 号：S562[农业科学—作物学]