Determination of Gossypol in Trace Level by Flow Injection Analysis with Chemiluminescence Detection  

作　　者：Bing Chun XUE Er Bao LIU 

机构地区：[1]College of Chemistry and Life Sciences, Tianjin Normal University, Tianjin 300074

出　　处：《Chinese Chemical Letters》2006年第1期57-60,共4页中国化学快报（英文版）

基　　金：financial support from the National Natural Science Foundation of China(Grant No.20075017)and from Tianjin Normal University.

摘　　要：This work reports the single-molecule detection of gossypol by flow injection analysis with chemiluminescence method. The method is based on the reaction of luminol with ferricyanid in sodium hydroxide medium sensitized by gossypol. Under the optimum conditions, the CL intensity is proportional to the concentration of gossypol over the range of 1.11×10^-17-2.78×10^-16 mol/L in acid solution and 8.00×10^-11-7.39×10^-8mol/L in neutral solution with correlation coefficients 0.9983 and 0.9905, respectively. The detection limits is 1.60×10^-18 mol/L （S/N=3）. The proposed method has been applied for the determination of the gossypol in cottonseeds and pharmaceutical formulations with satisfactory results.This work reports the single-molecule detection of gossypol by flow injection analysis with chemiluminescence method. The method is based on the reaction of luminol with ferricyanid in sodium hydroxide medium sensitized by gossypol. Under the optimum conditions, the CL intensity is proportional to the concentration of gossypol over the range of 1.11×10^-17-2.78×10^-16 mol/L in acid solution and 8.00×10^-11-7.39×10^-8mol/L in neutral solution with correlation coefficients 0.9983 and 0.9905, respectively. The detection limits is 1.60×10^-18 mol/L （S/N=3）. The proposed method has been applied for the determination of the gossypol in cottonseeds and pharmaceutical formulations with satisfactory results.

关 键 词：Single-molecule detection CHEMILUMINESCENCE flow injection gossypol. 

分 类 号：R284[医药卫生—中药学] R917[医药卫生—中医学]