Study of Growth Mechanism of Lysozyme Crystal by Batch Crystallization Method  

作　　者：Hai Liang CUI Yong YU Wan Chun CHEN Qi KANG 

机构地区：[1]National Microgravity Lab, Institute of Mechanics, Chinese Academy of Science, Beijing 100080 [2]Institute of Physics, Chinese Academy of Science, Beijing 100080

出　　处：《Chinese Chemical Letters》2006年第1期101-104,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.10472127 and No.10432060);Knowledge Innovation Program of Chinese Academy of Sciences(KSCX2-SW-322,KICX2-SW-L05).

摘　　要：The lysozyme crystals were made by batch crystallization method and the distribution of aggregate in solution were measured by dynamic light scattering. The results showed that the dimension of aggregate increased with the increase of the concentration of lysozyme and NaCl, lysozyme molecules aggregated gradually in solution and finally arrived at balance each other. The higher the concentrations of lysozyme and NaCl were, the faster the growth rate of （110） face was. The growth rates of lysozyme crystal were obtained by a Zeiss microscope, and the effective surface energy （a） of growing steps were calculated about 4.01×10^-8s J·cm^-2 according to the model of multiple two-dimensional nucleation mechanism.The lysozyme crystals were made by batch crystallization method and the distribution of aggregate in solution were measured by dynamic light scattering. The results showed that the dimension of aggregate increased with the increase of the concentration of lysozyme and NaCl, lysozyme molecules aggregated gradually in solution and finally arrived at balance each other. The higher the concentrations of lysozyme and NaCl were, the faster the growth rate of （110） face was. The growth rates of lysozyme crystal were obtained by a Zeiss microscope, and the effective surface energy （a） of growing steps were calculated about 4.01×10^-8s J·cm^-2 according to the model of multiple two-dimensional nucleation mechanism.

关 键 词：Protein crystal growth rate dynamic light scattering lysozymc. 

分 类 号：Q55[生物学—生物化学] Q51