SBA-15 Modified Carbon Paste Electrode for Rapid cTnI Detection with Enhanced Sensitivity  

作　　者：Nong Yue HE Hui Shi GUO Di YANG Chun Rong GU Ji Nan ZHANG 

机构地区：[1]State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096 [2]Institute of Cardiovascular Disease, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029 [3]Key Laboratory of Green-Packaging and Application of Bionanotechnology of Hunan Province, Zhuzhou Institute of Technology, Zhuzhou 412008

出　　处：《Chinese Chemical Letters》2006年第2期235-238,共4页中国化学快报（英文版）

摘　　要：A novel electrochemical immunoassay for cardiac troponin Ⅰ （cTnI） combining the concepts of the dual monoclonal antibody ＂sandwich＂ principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode （SBA-MCPE） is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.A novel electrochemical immunoassay for cardiac troponin Ⅰ （cTnI） combining the concepts of the dual monoclonal antibody ＂sandwich＂ principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode （SBA-MCPE） is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.

关 键 词：Cardiac troponin Ⅰ SBA-15 mesoporous materials carbon paste electrode rapid quantitative immunoassay anodic stripping voltammetric detection NANO-GOLD sensitivity. 

分 类 号：R972.1[医药卫生—药品] R917[医药卫生—药学]