可见光及紫外光分光光度法测定溪黄草总黄酮  

DETERMINATION OF FLAVONOID IN RABDOSIA LOPHANTHOIDES BY UV AND VIS SPECTROPHOTOMETRY

作　　者：黎彧 吴志谊[3] 吴川[3] 马宝娟[3] 吴风兰[3] 

机构地区：[1]中山大学化学与化学工程学院 [2]广东轻工职业技术学院轻化系广东广州,510300 [3]广东轻工职业技术学院轻化系

出　　处：《精细石油化工》2006年第1期51-53,共3页Speciality Petrochemicals

摘　　要：以芦丁为参照品比较了可见光及紫外分光光度法测定溪黄草总黄酮的方法。在可见光分光光度法中,硝酸铝作显色剂,测量波长为510 nm。紫外分光光度法,测量波长为359 nm。结果表明,紫外分光光度法是一种较理想的测定方法。Total flavonoid content in Rabdosia lophanthoides was determined by VIS and UV Spectrophotometry, both with rutin as reference sample. In VIS spectrophotometric analysis, Al（NO3）3 was used as the revealing agent and the measurement was conducted at 510 nm. In UV spectrophotometric analysis ,the measurement was canducted at 359 nm. The comparation of two methods indicated that UV spectrophotometry was a better method for the determination of the total flavonoid content in Rabdosia lophanthoides.

关 键 词：溪黄草 总黄酮 可见光分光光度法 紫外分光光度法 

分 类 号：O657.3[理学—分析化学]