常用十字花科药用植物中芥子碱的分布研究  

Study of Distribution of Sinapine in Commonly Used Crude Drugs from Cruciferous Plants

作　　者：刘丽芳[1] 王宇新[1] 李海燕[1] 及莹[1] 

机构地区：[1]中国药科大学现代中药教育部重点实验室,江苏南京210038

出　　处：《色谱》2006年第1期49-51,共3页Chinese Journal of Chromatography

基　　金：国家药典委员会资助项目

摘　　要：十字花科植物中的芥子碱是一种非常有价值的天然抗氧剂,在抗衰老药物的研究中受到了许多学者的重视。为了考察芥子碱在我国药用植物中的分布情况,采用反相高效液相色谱法测定了我国常用的4种十字花科药用植物白芥子、莱菔子、葶苈子、黄芥子中该成分的含量。采用的色谱柱为A lltim a Pheny l柱(250mm×4.6mm i.d.,5μm);流动相:3%醋酸溶液和乙腈的混合溶液,梯度洗脱;检测波长:326nm;柱温:25℃。该法快速,准确度高,重现性好,方法的建立不仅为上述4种药材的质量监测提供了有效的定量分析方法,而且对于芥子碱的深入研究具有重要意义。Sinapine, an important natural antioxidant, is often obtained from cruciferous plants. Many scientists are interested in it because of its great potential in the field of antiageing drugs. Thus, the distribution of sinapine in 4 commonly used crude drugs from cruciferous plants （Semen Sinapis Albae, Semen Brassicae Junceae, Semen Raphani and Semen Lepidii） was investigated. The determination was performed by reversed-phase high performance liquid chromatography （RP-HPLC） using an Alltima Phenyl column （250 mm × 4.6 mm i. d. , 5μm）. A gradient elution with mobile phase consisting of （A） 3% acetic acid in water and （B） acetonitrfle was used. The detection wavelength was set at 325 nm and the column temperature was kept at 25 ℃. It is an accurate, rapid and reproducible method. The results are informative for the research of a quality control tool for these natural drugs.

关 键 词：高效液相色谱法 芥子碱 抗氧化剂 白芥子 黄芥子 莱菔子 葶苈子 

分 类 号：O658[理学—分析化学]