联用色谱及多组分光谱相关色谱用于当归补血汤与单味药成分的比较研究  

Comparison Studies of Constituents Between Dangguibuxuetang and Its Single Medicines via Hyphenated Chromatography and Multicomponent Spectral Correlative Chromatography

作　　者：王亚丽[1] 梁逸曾[1] 胡芸[2] 李博岩[1] 曾仲大[1] 徐抒平[3] 

机构地区：[1]中南大学中药现代化研究中心 [2]日本关西学院大学理工学部化学系 [3]吉林大学超分子结构与材料教育部重点实验室,长春130023

出　　处：《高等学校化学学报》2006年第2期222-226,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20235020);甘肃省攻关课题(批准号:GS022-A43-138)资助

摘　　要：利用HPLC-DAD提供的二维化学数据信息,通过多组分光谱相关色谱方法及化学计量学分辨技术,对相同试验条件下所得的当归补血汤及其单味药的色谱组分进行了比较和归属分析.结果表明,在当归补血汤色谱响应值较高的21个色谱峰中,8个色谱峰的组分来自当归,6个色谱峰的组分来自黄芪,7个色谱峰的组分同时来源于当归和黄芪.其中一些色谱峰簇中的其它组分发生了消长变化,既有新物质的产生,又有原有化学成分的消失,说明中药复方配伍的药效物质基础不仅是各单味药化学成分的总和,还有单味药在复方配伍过程中各种成分的相互作用.本方法为中药复方与单味药化学组分的比较及归属分析,尤其对重叠色谱峰簇的组分比较分析提供了方便、快捷及可行的研究方法.In this study, a compound medicine of Dangguibuxuetang and its two involved single medicines of Danggui （ Radix Angelica sinensis） and Huangqi （ Radix Astragali） were compared for their relevant constituents after the combination of hyphenated chromatography and muhicomponent spectral correlative chromatography, based on the two-dimensional spectrochromatographic fingerprints of the three medicinal objects obtained from HPLC-DAD. It was found that of twenty-one high peaks in Dangguibuxuetang, eight originate from Radix An- gelica sinensis, six from Radix Astragali, and seven from both Radix Angelica sinensis and Radix Astragali. A little of other components in the investigated overlapping peaks varied. Moreover, new chemical ingredients appeared just in spectrochromatographic fingerprints of Dangguibuxuetang, and some existing in those of single medicines contrarily disappeared after the compatibility, as opposite in Radix Angelica sinensis and Radix Astragali. It suggested that there are certain interactions of the chemical ingredients in compound medicine besides their total sum effect of single medicines. This proposed approach was quite convenient and reliable to make a quick comparison and analysis of the relevant constituents in spectrochromatographyic fingerprints of the compound and single medicines, especially for the comparison of two overlapping peaks.

关 键 词：联用色谱 多组分光谱相关色谱 当归补血汤 单味药 组分比较 

分 类 号：O657[理学—分析化学]