分子信标研究5-氟尿嘧啶对鼻咽癌p33^(HNE1)细胞  

Investigation of the Effect of 5-FU on p33^(ING1) mRNA Level Based on Molecular Beacon

作　　者：王炜[1] 刘斌[1] 李军[1] 王柯敏[1] 谭蔚泓[1] 唐红星[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室湖南省生物纳米技术研究中心湖南大学生物医学工程中心,长沙410082

出　　处：《高等学校化学学报》2006年第2期268-270,共3页Chemical Journal of Chinese Universities

基　　金：国家重点基础研究发展规划(批准号:2002CB513110);国家自然科学基金重点项目(批准号:20135010);国家自然科学基金青年基金(批准号:20305006);湖南省科研计划重点项目(批准号:0399Y1006)资助

摘　　要：In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells,p33ING1 mRNA level in cellular total RNA was detected quantitatively based on MB assay.During the operation,the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33ING1 were measured in vitro.The results were as follows: p33ING1 mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time.The MTT results also proved that high expression of p33ING1 mRNA can increase cell’s sensitivity to chemical drug 5-FU.The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells, p33^ING1 mRNA level in cellular total RNA was detected quantitatively based on MB assay. During the operation, the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33^ING1 were measured in vitro. The results were as follows： p33^ING1 mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time. The MTr results also proved that high expression of p33^ING1 mRNA can increase cellg sensitivity to chemical drug 5-FU. The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.

关 键 词：分子信标 P33^ING1 mRNA 5-FU 鼻咽癌 药物敏感性 

分 类 号：O629.74[理学—有机化学]