玉竹提取物A对小鼠免疫性肝损伤的保护作用  

Protective effects of extraction A of polygonatum odoratum on immune hepatic injury of mice

作　　者：赵良中[1] 赵良化 佟伟[1] 李宏伟[1] 潘兴瑜[1] 

机构地区：[1]锦州医学院免疫与病原生物学教研室,辽宁省锦州市121001 [2]庄河市第四人民医院内科,辽宁省庄河市116400

出　　处：《中国临床康复》2006年第3期99-101,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨玉竹提取物A对小鼠免疫性肝损伤的保护作用,探讨其发挥此作用的可能的机制。方法:①实验于2004-06/10在锦州医学院免疫实验室完成。选用健康雄性昆明小鼠50只,普通级。将50只健康雄性昆明小鼠随机分为5组:正常对照组、模型对照组、玉竹提取物A0.5g/kg组、玉竹提取物A1g/kg组、玉竹提取物A2g/kg组,每组10只。②玉竹提取物A是玉竹的干燥根茎经水醇法提取而得。③正常对照组和模型对照组小鼠腹腔注射生理盐水,玉竹提取物A0.5,1,2g/kg组小鼠分别腹腔注射相应浓度的玉竹提取物A,每次每只0.5mL,3次/d,连续注射4d。最后一次注射后8h,除正常对照组注射生理盐水外,其余各组均尾静脉注射20mg/kg的刀豆蛋白A0.5mL,制备免疫性肝损伤模型。④注射刀豆蛋白A8h后摘眼球取血低温保存,取肝待行光镜检查,以观察肝脏组织病理学改变(常规苏木精-伊红染色),取脾待行淋巴细胞增殖实验(采用四甲基偶氮唑盐法)和流式细胞术以检测T淋巴细胞亚群。各组小鼠血清谷丙转氨酶活性检测按购自南京建成生物研究所的谷丙转氨酶试剂盒说明进行。⑤组间计量资料差异比较采用方差分析。结果:昆明小鼠50只均进入结果分析。①肝脏组织病理学改变:模型对照组可见明显免疫性肝损伤病理改变。玉竹提取物A3个实验组小鼠肝损伤病理改变均不同程度的好于模型对照组。②玉竹提取物A对血清谷丙转氨酶活性的影响:模型对照组血清谷丙转氨酶活性明显高于正常对照组(t=13.8,P<0.01),玉竹提取物A3个实验组血清谷丙转氨酶活性低于模型对照组,尤以玉竹提取物A2g/kg组与模型对照组差异最明显(t=5.62,P<0.01),且存在剂量依赖关系。③玉竹提取物A对T淋巴细胞转化增值的影响:刀豆蛋白A刺激体外培养的各组脾淋巴细胞,模型对照组的刺激指数明显高于正常对照组(t=9.02,P<0.01),玉竹提取物AAIM： To investigate the protective effect of extraction A of polygonatum odoratum （EA-PAOA） on the immune hepatic injury in mice and study its protective mechanisms. METHODS：（1）The experiment was carried out in the immunological laboratory of Jinzhou Medical College from June to October 2004. Fifty Kunmlng mice （common grade） were selected and randomized into five groups with ten mice in each group： normal control group, model control group, test 1 （EA-PAOA 0.5 g/Kg） group, test 2 （EA-PAOA 1 g/Kg） group and test 3 （EA-PAOA 2 g/Kg） group. （2）The EA-PAOA was extracted from dry root and petiol with water-ethanol method （30 mL/L ethanol）. （3）Saline was injected into abdominal cavity of mice in the normal control group and the model control group. Mice in the test 1, test 2 and test 3 groups were given with the corresponding EA-PAOA through intraperitoneal injection, respectively, once 0.5 mL into every mice, 3 times per day, for 4 days. Eight hours after the last injection, the mice in the model control and three test groups, respectively, received intravenous injections of concanavalin A （20 mg/kg） 0.5 mL, except these in the normal control group were injected with saline. Then the immune liver injury mice were prepared. （4）Blood gained from the picked eyeball were collected at eight hours after intravenous injections of concanavalin A, and then kept in hypothermia. The liver was reserved for routine light microscopy so as to observe the pathological changes of liver tissue （hematoxylin-eosin staining）. The spleen was aimed to do lymphocyte proliferation experiment and T-cell proliferactive activity tests with MTT, The level of alanine aminotransferase was tested with Kit made from Nanjing Jiancheng Bioengineering Institute. （5））The analysis of variance was applied to the compare of computation data. RESULTS： Totally 50 Kumming mice were involved in the result analysis. （1） Pathological change of liver tissue： Obvious immune hepatic injury appea

关 键 词：肝疾病 中药疗法 免疫性疾病 中草药 T淋巴细胞亚群 玉竹提取物A 免疫性肝损伤 保护作用 小鼠 

分 类 号：R259.75[医药卫生—中西医结合]