佐剂性关节炎大鼠细胞因子和皮质醇水平与茜草总蒽醌的干预作用  

Interventional effect of total anthraquinone of Rubia cordifolia·L on cytokine and cortisol levels in adjuvant arthritis rats

作　　者：许兰芝[1] 胡庆伟[1] 冷平[1] 

机构地区：[1]潍坊医学院药理教研室,山东省潍坊市261042

出　　处：《中国临床康复》2006年第3期116-117,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察茜草总蒽醌对佐剂性关节炎大鼠的抗炎作用及其与细胞因子和皮质醇关系,并与地塞米松进行阳性对照。方法:实验于2001-10-01/12-15在潍坊医学院药理实验室完成。①取成年SD大鼠35只,随机分为5组(n=7):对照组,模型组,地塞米松组,总蒽醌60,120mg/(kg·d)组。后4组大鼠于右足垫皮内注射弗氏完全佐剂0.05mL制备佐剂性关节炎大鼠模型。②造模后第17天开始,地塞米松组腹腔注射地塞米松2.5mg/(kg·d),总蒽醌60,120mg/(kg·d)组分别灌胃茜草总蒽醌60,120mg/(kg·d),对照组和模型组灌胃等容积的生理盐水,连用10d。③观察大鼠给药前后左足爪的肿胀度和全身病变评分,并用放射免疫法测定给药后血清中白细胞介素1,2,6及肿瘤坏死因子和皮质醇的水平。结果:35只大鼠进入结果分析。①地塞米松组,总蒽醌60,120mg/(kg·d)组大鼠给药后左足爪的肿胀度、全身病变评分均低于模型组(P<0.01),地塞米松组效果优于其他两组(P<0.01)。②地塞米松组,总蒽醌60,120mg/(kg·d)组大鼠血清中白细胞介素1,2,6和肿瘤坏死因子水平均低于模型组(P<0.01),地塞米松组作用最明显,总蒽醌60mg/(kg·d)组作用最弱。各组皮质醇水平比较无差异(P>0.05)。结论:①茜草总蒽醌能显著减轻佐剂性关节炎大鼠的炎症反应,其作用可能与降低血清中白细胞介素1,2,6,和肿瘤坏死因子水平有关,而与垂体-肾上腺皮质轴无关。②茜草总蒽醌的效果比地塞米松弱。AIM： To observe the anti-inflammatory effect of total anthraquinone of Rubia eordifolia ·L （TAR） in adjuvant arthritis rats and its relation with cytokine and cortisol, and perform positive control with desamethasone. METHODS： The experiment was conducted at the laboratory of pharmacology of Weifang Medical College from October 1# to December 15^th 2001. （1）thirty-five adult SD rats were selected and randomly divided into 5 groups （n=7）： Control group, model group, desamethasone group and 60 and 120 mg/kg per day total anthraquinone group. 0.05 mL Freund＇s complete adjuvant was injected into right footpad of rats in the later 4 groups to establish rat models with adjuvant arthritis. （2）From the 17^th day after building models, 2.5 mg/kg per day desamethasone was injected into rats of desamethasone group through intraperitoneal injection. 60 and 120 mg/kg per day total anthraquinane of TAR were given with gastric peffusion in the 60 and 120 mg/kg per day total anthraquinone group, respectively. The same volume saline was given with gastric perfusion into rats of the control group and the model group for 10 days. （3）Degree of swelling of left foot claw, score on lesion of total body were observed before and after administration in rats. Levels of interleukin 1, 2 and 6, tumor necrosis factor （TNF） and cortisol in serum were detected with radio-immunity method after administration. RESULTS： Thirty-five rats were involved in the result analysis, （1）The degree of swelling of left foot claw and score on lesion of total body in rats after administration in the desamethasone group and the 60 and 120 mg/kg per day total anthraquinone groups were lower than those in the model group （P〈0.01）, and the effect in the desamethasone group was better than that in the other two groups （P 〈 0.01）. （2）The levels of interleukin 1, 2 and 6 and the TNF was lower in the desamethasone group and the 60 and 120 mg/kg per day total anthraquinone groups than those in the model group

关 键 词：茜草 关节炎 免疫抑制 细胞因子类 佐剂性关节炎 细胞因子 皮质醇 茜草总蒽醌 干预作用 

分 类 号：R259.932.2[医药卫生—中西医结合]