双根清脑煎剂对血管性痴呆大鼠模型行为学的影响(英文)  

Effects of shuanggen cephalocathartic decoctum on model rats with vascular dementia in behavioristics

作　　者：吴颢昕[1] 蔡建伟[1] 向红兵[2] 林靓[1] 

机构地区：[1]南京中医药大学中医基础理论教研室,江苏省南京市210029 [2]南京中医药大学解剖教研室,研究生江苏省南京市210009

出　　处：《中国临床康复》2006年第3期180-182,共3页Chinese Journal of Clinical Rehabilitation

基　　金：江苏省中医药局资助项目(H-013)~~

摘　　要：背景:血管性痴呆是指由缺血性或出血性中风或缺血缺氧性脑损害导致的以认知损害为特征的综合征,中医学将其归属于“愚痴”、“痴呆”、“神呆”、“善忘”等范畴。由于痴呆造成患者记忆、认知等方面的障碍,严重影响老年人的身心健康和生活质量。目的:观察双根清脑煎剂对血管性痴呆大鼠学习、记忆能力及空间分辨能力的影响。设计:以实验动物为观察对象的随机对照观察。单位:南京中医药大学基础医学院中医基础理论教研室。材料:实验于 2004-01/02在南京中医药大学中医基础理论教研室藏象实验室完成。选择雄性 SD 大鼠 40只,随机分为假手术组、模型组、模型加中药组及模型加西药组,每组 10只。所有大鼠实验前适应性喂养一周,实验前和实验时自由饮水和摄食。药品与试剂为双根清脑煎剂和尼莫通片,主要实验仪器为 DTT-2型跳台仪和 SM G-2型水迷宫实验装置。方法:在无菌状态下,取大鼠左心室内血,37℃温箱内干燥,研碎后200μm 筛孔过筛制成栓子,应用时按栓子 1m g 加生理盐水 0.3m L 配制,摇匀成混悬液。以短暂夹闭颈总动脉,并从颈外动脉逆行插管注入含有栓子的混悬液进入颅内制作多发性脑梗塞模型。假手术组中空细管只插到颈总动脉分叉处,不注入任何液体。实验前模型组和假手术组给予正常饮料喂养,其他各组除正常饲料喂养外,模型加中药组同时给予双根清脑煎剂 4g/(kg·d),西药组给予尼莫通 2m g/(kg·d),每日灌服 1次,连续 15d 为 1个疗程。各组大鼠均于常规喂养及给药后第 9天进行被动回避性跳台实验,记录大鼠受到电刺激后跳上橡皮垫的反应时间及 3m in 内跳下平台遭电击的错误次数,作为学习指标。于第 10天再次测试,先将大鼠置于箱内适应 3m in,再将其置于橡皮垫上,记录其第 1次跳下橡皮垫的潜伏期,并记录 3m in 内跳下橡皮垫次�BACKGROUND： Vascular dementia is a syndrome characterized by cognitive impairment, which is induced by ischemic or hemorrhagic apoplexy or ischemic-anoxic brain injury. It is assigned to ＂imbecility＂, ＂dementia＂, ＂sluggish consciousness＂, ＂susceptible to forget things＂ etc. by traditional Chinese medicine. Impairments of memory and cognition etc. caused by dementia seriously affect psychosomatic health and quality of life of elderly people. OBJECTIVE： To observe the effects of shuanggen cephalocathartic deeoctum on learning, memory and spatial discrimination ability of vascular dementia rats. DESIGN： Randomized control trail taking experimental animals as objects. SETTING： Teaching and Research Section of Basic Theory of Traditional Chinese Medicine, Preelinical Medicine College, Nanjing University of Traditional Chinese Medicine. MATERIALS： The experiment was performed in Laboratory of Viscerastate of Teaching and Research Section of Basic Theory of Traditional Chinese Medicine, Preclinieal Medicine College, Nanjing University of Traditional Chinese Medicine, from January to February 2004. A total of 40 male SD rats were selected and randomly divided into sham operation group, model group, model plus traditional Chinese medicine group and model plus Western medicine group, with 10 in each group. Before the experiment, all the rats were adaptively fed for one week, free drinking and eating before and during the experiment. Medicine and reagent are shuaongen cephalocathartic decoctum and Nimotop tablet, and main experimental apparatuses are DTT-2 jumping apparatus and SMG-2 water maze apparatus. METHODS： Blood was collected from left ventricle under aseptic condition, and dried in incubator at 37℃, after trituration, it was sifted with sieve of 200μm meshes and embolus was prepared. 1 mg embolus and 0.3 mL physiological saline was mixed and shaken up into suspension. Common carotid artery was temporarily occluded with a vascular clamp, and external carotid artery was retrogr

关 键 词：痴呆 血管性 行为 大鼠 Sprague-Dawsley 血管性痴呆 双根清脑煎剂 

分 类 号：R277.791.3[医药卫生—中医学]