Study on the interaction of anticancer drug mitoxantrone with DNA by fluorescence and Raman spectroscopies  

作　　者：汤玲娟 孙真荣 郭建宇 王祖赓 

机构地区：[1]Key Laboratory of Optical and Magnetic Resonance Spectroscopy (East China Normal University)

出　　处：《Chinese Optics Letters》2006年第2期101-104,共4页中国光学快报（英文版）

基　　金：This work was supported by the National Natural Sci-ence Foundation of China (No. 10234030 and 10374030)the National Key Project for Basic Research of China (No. 1999075204) the Program for New Century Excel-lent Talents in University (NCET-04-0420) the Shang-hai Science and Technology Committee (No. 036105019)and the Twilight Project sponsored by Shanghai Edu-cation Committee (No. 03SG23).

摘　　要：Mitoxantrone, a clinically useful antitumour antibiotic for leukaemia and breast cancer, has received more attentions. In this paper, the interaction between mitoxantrone and calf thymus DNA is investigated by Raman and fluorescence spectroscopies, and the binding site of mitoxantrone to calf thymus DNA is explored. The results showed that mitoxantrone interacts with calf thymus DNA bases by the intercalation of anthracycline into the base pair plane of adenine （A） and thymine （T）, and it results in the disruption of the hydrogen bonds between calf thymus DNA bases, and thus the calf thymus DNA double-strand can be disrupted into the B-form DNA double-strand scgments.Mitoxantrone, a clinically useful antitumour antibiotic for leukaemia and breast cancer, has received more attentions. In this paper, the interaction between mitoxantrone and calf thymus DNA is investigated by Raman and fluorescence spectroscopies, and the binding site of mitoxantrone to calf thymus DNA is explored. The results showed that mitoxantrone interacts with calf thymus DNA bases by the intercalation of anthracycline into the base pair plane of adenine （A） and thymine （T）, and it results in the disruption of the hydrogen bonds between calf thymus DNA bases, and thus the calf thymus DNA double-strand can be disrupted into the B-form DNA double-strand scgments.

关 键 词：Antibiotics DNA Drug products FLUORESCENCE Hydrogen bonds ONCOLOGY Raman spectroscopy Tumors 

分 类 号：R96[医药卫生—药理学]