腹膜透析对转化生长因子β_1在残存肾中表达的影响  

Effectofperitonealdialysisontransforminggrowthfactors┐β1inremnantkidney

作　　者：郑智华[1] 叶任高[1] 余学清[1] 许乃贵[1] 陈永雄[1] 汪涛[1] 

机构地区：[1]中山医科大学肾脏研究所

出　　处：《中华肾脏病杂志》1996年第5期262-264,共3页Chinese Journal of Nephrology

摘　　要：探讨清除循环中毒素后生长因子和残存肾小球的改变。方法采用分子杂交技术和组织病理分析方法在肾次全切除的残存肾大鼠模型上研究腹膜透析（ＰＤ）对转化生长因子β１（ＴＧＦ－β１）ｍＲＮＡ在残存肾中表达的影响和肾小球硬化的变化。分模型组和ＰＤ组，第８周ＰＤ组开始作腹膜透析至第１２周。分别在第７周和１２周各组杀检６只作肾病理检查（ＨＥ．ＰＡＳ染色）和ＴＧＦ－β１ｃＤＮＡ缝隙点样杂交。结果在实验的第７周两组间肾小球硬化指数（ＧＳＩ）及ＴＧＦ－β１ｍＲＮＡ表达均无显著性差异。第十二周，ＰＤ组的ＧＳＩ及ＴＧＦ－β１ｍＲＮＡ表达均显著低于模型组。ＧＳＩ改变程度与ＴＧＦ－β１ｍＲＮＡ表达丰度变化呈明显正相关（ｒ＝０．６５Ｐ＜０．０５）。bjectiveToclarifythechangesofremnantglomeruliandtransforminggrowthfactors-β1(TGF-β)aftertheclearanceofcirculatingtoxins.MethodsSlot-blothybridizationwithcDNAprobeandhistopathologicalanalysiswereusedwithsubtotalnephrectomyratmodeltoevaluatethechangeofglomerularscleriosisandtheeffectofperitonealdialysis(PD)ontheexpressionofTGF-β1mRNAinremnantkidney;RatsweredividedtoPD、modelandcontrolgroup;PDgroupwasrecieveddialysisfor4weeksfromeighthweek;PathologicalandTGF-β1cDNAslot-blothybridizationwereperformedinseventhandtwelfthweekrespectively.ResultsInseventhweek,therewasnosignificantdiferenceofglomerularscleriosisindex(GSI)andTGF-β1mRNAexpressionbetweenPDandmodelgroup;Intewlfthweek,GSIandTGF-β1mRNAexpressionofPDgroupweresignificantlowerthanthatofmodelgroup;TherewasapositivecorrelationbetweenthedegreeofGSIandtheabundanceofTGF-β1mRNAex┐pression.ConclusionPDcanreduceobviouslytheexpressionofTGF-β1mRNAandatenuateglomerularsclerio-sis.

关 键 词：腹膜透析 转化生长因子 β1 残存肾 

分 类 号：R692.505[医药卫生—泌尿科学] R459.5[医药卫生—外科学]