氯丙烯中毒大鼠神经电生理时间效应关系  

Study on time dependent changes of nerve electrophysiology induced by allyl chloride in rats

作　　者：王青山[1] 朱英建[1] 张利平[1] 郭新[1] 谢克勤[1] 

机构地区：[1]山东大学公共卫生学院毒理学研究所,济南250012

出　　处：《中国公共卫生》2006年第2期157-159,共3页Chinese Journal of Public Health

基　　金：国家973基金项目(2002CB512907);国家自然科学基金项目(30271138)

摘　　要：目的探讨氯丙烯亚慢性中毒大鼠坐骨神经的电生理指标改变时间效应关系。方法选用Wistar雄性大鼠,以200 mg/(kg.bw)剂量经口灌胃,每周3次,在第0,3,6,9,12周,用电生理仪分别测定相应对照组与染毒组大鼠坐骨神经传导速度、复合动作电位峰峰值、潜伏期、时程、阈强度及最大刺激强度等指标。结果电生理各指标变化随时间和步态评分呈进行性加重,坐骨神经传导速度从第6周和步态评分2分开始降低,与对照组相比差异有统计学意义(P<0.05);复合动作电位峰峰值从第12周和步态评分4分开始降低,与对照组相比差异有统计学意义(P<0.05);,其它各指标均从第9周和步态评分3分开始逐渐增大,与对照组相比差异亦有统计学意义(P<0.05)。结论氯丙烯引起的坐骨神经电生理各指标的改变存在时间效应关系,其中以传导速度改变的最早、最为敏感,为氯丙烯中毒的早期诊断提供一定的依据。Objective To study the time dependent changes of sciatic nerve electmphysiology caused by allyl chloride in wistar rats. Methods 90 Male Wistar rats weighting 180-220 g were divided randomloy into nine groups, i. e. experimental groups for 3, 6, 9, 12 weeks and control group for 0, 3, 6, 9, 12 weeks, each 10 animals. The animals in experimental groups were treated with allyl chloride dissolved in cornoil （200 mg/kg ip 3 days/week）by gavage. Eleetmphisieal indexes of each group were determined on the corresponding deadline. The indexes included measurements of sciatic nervous conduct velocity, action potential amplitude, latency, wave length, threshold intensity and max stimulate intesity. Results All indexes of electrophysiology were changed gradually in rats treated by aUyl chloride. The nervous conduct velocity significantly decreased as compared with the control rats, in 6-week experimental group（P〈0.05）, 9 -week experimental group （ P 〈 0.01 ）, 12-week experimental group（ P〈0.01 ） and the decrease（ P〈 0.01） accompanied 2, 3,4 gait scores; amplitude decreased as compared to the control rats（ P〈0.05 ）, in 12 - week experimental group and the decrease（ P〈0.01 ） accompanied 4 gait score. While the rest indexes were significantly increased（P 〈 0.05）in 9 -week experimental group, 12 -week experimental group and the increase（P〈0. 01） accompanied 3, 4 gait scores. Conclusion The time dependent changes in eleetrophysiology of wistar rats sciatic nerves caused by allyl chloride has occured. The nervous conduct velocity may be used as the most sentitive indicator to the early diagnosis for the allyl chloride toxieosis.

关 键 词：电生理 动作电位 神经传导速度 氯丙烯中毒 大鼠 时间效应 

分 类 号：R595.4[医药卫生—内科学] R99[医药卫生—临床医学]