饮食诱导肥胖大鼠sTNFR2与胰岛素抵抗关系  

Study on relationship between sTNFR2 and insulin resistance in DIO rats

作　　者：王艳[1] 姚兴家[1] 郑琳琳[2] 翟玲玲[1] 吴辉[3] 贾丽红[1] 刘玉芬[1] 

机构地区：[1]中国医科大学公共卫生学院儿少卫生教研室,沈阳110001 [2]辽宁省丹东市辽东学院医学院 [3]中国医科大学公共卫生学院社会医学教研室

出　　处：《中国公共卫生》2006年第2期196-197,共2页Chinese Journal of Public Health

摘　　要：目的探讨饮食诱导肥胖(DIO)大鼠血清可溶性肿瘤坏死因子受体2(sTNFR2)与胰岛素抵抗(IR)的关系。方法采用双抗体夹心ELISA法检测大鼠血清sTNFR2水平,放免法检测血清肿瘤坏死因子-α(TNF-α)、空腹胰岛素(FINS)水平。结果DIO大鼠血清sTNFR2、TNF-α水平显著高于对照组和饮食诱导肥胖抵抗(DIO-R)大鼠(P<0.01),DIO大鼠血清sTNFR2、TNF-α水平均与胰岛素敏感指数(ISI)呈负相关(P<0.05)。结论sTN-FR2在肥胖相关的IR发生中可能起重要作用。Objective To study the relationship between serum soluble tumor necrosis factor receptor 2 （sTNFR2）and insulin resistance（IR）in diet-induced obesity （DIO） rats. Methods The level of serum sTNFR2 was determined by enzyme linked immunosorbent assay（ELISA）. The concentrations of serum tumor necrosis factor α（TNF-α） and fasting insulin （FINS） were measured by radioimmunoassay. Results The levels of sTNFR2 and TNF-α of DIO rats were significantly higher than those of the controls and diet-induced obesity resistant（DIO-R） rats（ P 〈 0.01 ）. Insulin sensitivity index（ISI） was in stongly negative correlation with the levels of serum sTNFR2 and TNF-α in DIO rats （ P〈0.05 ）. Conclusion sTNFR2 may play an important role in IR related to obesity.

关 键 词：肥胖 胰岛素抵抗 肿瘤坏死因子受体 肿瘤坏死因子-Α 饮食诱导 

分 类 号：R589.2[医药卫生—内分泌] R587[医药卫生—内科学]