微波消解原子荧光光谱法测定生物样品中砷汞  

AFS DETERMINATION OF ARSENIC AND MERCURY IN BIOLOGICAL SAMPLES——DIGESTION OF SAMPLE BY MICRO WAVE HEATING

作　　者：邬春华[1] 吕元琦[1] 袁倬斌[1] 

机构地区：[1]中国科学院研究生院化学与化学工程学院,北京100049

出　　处：《理化检验（化学分册）》2006年第1期41-42,45,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金："九五"国家攀登计划中药现代化关键问题基础研究中的安宫牛黄丸课题的资助项目;国家自然科学基金(29675022;29875027);"九五"国家重点科技攻关项目(96-A23-01-06)的资助

摘　　要：采用微波消解样品预处理技术和原子荧光光谱法，测定了生物样品中砷和汞的含量。确定了微波消解样品的条件，优化了仪器的最佳工作参数。砷和汞的含量分别在-～20μg·L^-1和0～16μg·L^-1范围内线性良好。砷和汞的回收率分别为100．5％和98．6％。砷和汞的检出限分别为0．21和0．06μg·L^-1。试验表明，该法快速简便、准确灵敏，应用于生物样品中砷、汞的测定，结果满意。Microwave heating of sample digestion was applied to the AFS determination of arsenic and mercury in biological samples. The sample was digested in polytetrafluoroethylene （PTFE） vessel with HNO3 and HClO4, and heated with microwave digestion instrument using the mode of gradient elevation of pressure. The sample solution obtained was made up to 25 mL with HCl（1＋9） and used as mother solution for AFS determination of arsenic and mercury. Optimum working parameters of the AFS instrument were given in detail. Linearity range for the determination of arsenic was found to be 0 to 20 μg · L^-1 , and for mercury to be 0-16 μg · L^-1 , with their detection limits of 0. 21 μg · L^-1 and 0. 06 μg · L^-1 respectively. In the analysis of biological sample, recoveries of 100. 5% for arsenic and 98. 6% for mercury were obtained, and RSD＇s obtained were less than 4%.

关 键 词：微波消解 原子荧光光谱法 砷 汞 

分 类 号：O657.31[理学—分析化学]