姜黄素抑制淋巴瘤细胞周期蛋白水平的研究  

Growth-inhibitory effects of curcumin on expressing of cycle related proteins in lymphoma cells

作　　者：吴青[1] 陈燕[1] 李新刚[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液病研究所

出　　处：《中国医院药学杂志》2006年第2期121-123,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金项目(编号:30271672)

摘　　要：目的：探讨姜黄素对淋巴瘤细胞株Raji细胞增殖和细胞周期的影响。方法：6．25～100μmol·L^-1的姜黄素分别处理Raji细胞12～60h后，MTT法检测Raji细胞的生长活性；流式细胞仪（FCM）检测细胞凋亡；蛋白印迹法（Western Blot）分析姜黄素作用前后，肿瘤细胞中周期蛋白E以及相应视网膜母细胞瘤基因Rb的表达。结果：姜黄素可呈时间及剂量依赖性抑制Raji细胞的增殖，抑制率为59．83％～91．46％。流式细胞仪检测Raji细胞，凋亡率为14．38％-61．18％。姜黄素刺激Raji细胞24h内周期蛋白E和视网膜母细胞瘤基因Rb蛋白表达显著增强（P〈0.05），但随着姜黄素浓度增高而表达下调，呈剂量依赖性下调。结论：姜黄素能干扰细胞周期进程，其作用机制为诱导Raji细胞的凋亡，下调周期蛋白E的表达，从而抑制其相应视网膜母细胞瘤基因Rb的表达，可能是其作用机制之一。OBJECTIVE To explore the effects of curcumin on cell cycle in B-NHL cell line Raji cells. METHODS Raji cells were treated with 6.25 -100 μmol·L^-1 curcumin for 12 -60 h and the growth inhibitory rates of Raji cells were measured by MTr assay. Cell apoptosis was inspected by flow cytometery（FCM）. The expression of cell cycle-associated cyclinE ,Rb,which was induced by curcumin, was determined by using Western blot analysis. RESULTS Curcumin selectively inhibited proliferation of Raji cell line in a dose-and time-dependent manner. Inhibitory rate was 59.83% - 91.46% （ P 〈 0.05） of Raji cells. The apoptosis rates were 14.38% -61.18% by flow cytometery （FCM）. The up regulation of cyelinE and Rb expression was observed within 24 h after the treatment with cureumin. The expression down regulation of dose-dependence was observed with the increasing of concentration. CONCLUSION Curcumin could disturb cell cycle progression of Raji cell and lead to apoptosis, down-regulation of cyclinE and Rb gene. that may play the important rule in the mechanism.

关 键 词：姜黄素 周期调控蛋白E 视网膜母细胞瘤基因Rb 凋亡 

分 类 号：R285.5[医药卫生—中药学]