高脂饮食对缺血-再灌流室颤阈值的影响及β受体阻滞剂的保护作用  

Influence of high-cholesterol diet on fibrillation threshold in rabbits during ischemia-reperfusion and the protective effect of β-blockers

作　　者：卜军[1] 何奔[1] 张存泰[2] 王琳[2] 

机构地区：[1]上海交通大学医学院附属仁济医院心内科,上海200127 [2]华中科技大学同济医学院附属同济医院心内科

出　　处：《中华急诊医学杂志》2006年第2期140-143,共4页Chinese Journal of Emergency Medicine

基　　金：上海市卫生局基金资助项目(044048)

摘　　要：目的探讨高脂饮食对家兔缺血再灌流(ischemiareperfusion,IR)室颤阈值的影响及β受体阻滞剂的保护作用。方法动物分为正常对照组、高脂组、高脂+普萘洛尔组、高脂+美托洛尔组。通过高脂饮食建造动脉粥样硬化模型,实验第10周行心肌IR,动态观察血清心肌酶(CK)活性,记录室速和室颤发生情况并测定室颤阈值。结果与正常对照组相比,高脂组实验10周时,血清总胆固醇明显升高(P<0.01),心肌IR期间血清CK活性明显增高,室速和室颤发作阵次的平均积分增高,室颤阈值显著下降(P<0.05)。普萘洛尔和美托洛尔对高脂家兔血脂未见显著性影响,但能显著降低心肌IR期间血清CK活性,增高室颤阈值,降低室性心律失常积分(P<0.05)。结论高脂饮食加重心肌IR损伤,降低室颤阈值。β受体阻滞剂对这种严重缺血情况下的组织损伤和电不稳定性具有保护作用。Objective To study the effect of high-cholesterol diet on fibrillation threshold during ischemiareperfusion （I/R） in rabbits and the protective effect of β-blockers. Methods Atheroselerosis model was established by high-cholesterol diet Rabbits were randomly divided into normal control group （NC group）, hyperlipidemic control group （HC group）, propranolol-treated group and metoprolol-treated group. After 10 weeks, is chemia-reperfusion was carried out in rats. Serum ereatinine kinase （CK） was examined, and values of ventricular tachycardia （VT） and ventricular fibrillation （VF） and VF threshold （VFT） were recorded during the I/R. Results At 10 weeks, serum total cholesterol level was significantly increased in HC group compared with NC group （P 〈 0.01 ）.Serum CK levels during reperfusion were significantly higher, values of VT and VF markedly higher, and VFT was significantly lower in HC group than those in NC group （ P 〈 0.05）. Administration of propranolol or metoprolol significantly reduced values of VT and VF, increased VFT, and decreased serum CK levels in hyperlipidemic rabbits （P 〈 0.05 ）. Conclusion Hyperlipidemia increases susceptibility to ischemia-reperfusion-induced arrhythmias and myocardial damage in rabbits, which could be attenuated by β-blockers.

关 键 词：高脂血症 再灌流损伤 心律失常 Β受体阻滞剂 保护作用 

分 类 号：R541.7[医药卫生—心血管疾病]