全反式维甲酸对HL-60细胞NF-kB、CyclinD1表达的影响  

Influence of all-trans retinoic acid on NF-kB and Cyclin D1 expresion of HL-60 cells

作　　者：李四强 侯忠赤[2] 宫超 田嘉玲 

机构地区：[1]解放军252中心医院血液科,河北保定071000 [2]北京大学医学部生理学系,北京100083

出　　处：《中国现代医学杂志》2006年第2期180-182,共3页China Journal of Modern Medicine

摘　　要：目的探讨全反式维甲酸(ATRA)对HL-60细胞周期、NF-kB和Cyclin D1表达的影响。方法采用不同浓度的ATRA作用于HL-60细胞,于48h后收集细胞,用流式细胞术分析HL-60细胞周期、NF-kB和Cyclin D1表达的变化。结果维甲酸组HL-60细胞G1期细胞比值升高,S期细胞比例下降,NF-kB、Cyclin D1表达均下降,其中10-7M、10-6M浓度组与对照组相比差异均有显著性(P<0.05),而10-8M浓度组却差异均无显著性(P>0.05);维甲酸10-6M浓度组NF-kB、CyclinD1的表达呈显著正相关(r=0.55,P<0.01,n=12)。结论全反式维甲酸(ATRA)通过下调NF-κB抑制CyclinD1活性表达,可能是其阻滞细胞周期诱导HL-60细胞分化的机制。【Objective】 Study the effects of ATRA on cell cycle and expression of CyclinD1 and NF-kB. 【Methods】 HL-60 cells were cultured in RPMI 1640 medium with NS and ATRA in the final concentrations of 10^-8 M, 10^-7 M and 10^-6 M, respectively. After culturing for 48 h, the expression of NF-kB, cells cycle and CyelinD1 were examined by FCM. 【Results】 Ratio of G1 cyelc cells in 10^-7 M and 10^-6 M groups significantly increased than those in control group （P〈0.05）, while there is no significance in 10^-8 M group （P〉0.05）, Ratio of S cycle cells in 10^-7 M and 10^-6 M groups significantly decreased than control group （P〈0.05）. The expression of NF-kB and CyclinD1 in 10^-7 M and 10^-6 M groups significantly decreased than those in eontml group （P〈0.05）. There was a good positive link between the expression of NF-kB and CyelinD1 in 10^- M groups （r=0.55, P〈0.01, n=12）. 【Conclusions】 All-trams retinoic acid could down-regulate CyelinD1 expression by restraining expression of NF-kB, then induce differentiation of HL-60 cells.

关 键 词：全反式维甲酸 核因子-KB 细胞周期素D1 

分 类 号：R331[医药卫生—人体生理学]