加味五子衍宗方及有效组分对记忆障碍小鼠中枢胆碱能系统的影响  

Effects of Jiawei Wuzi Yanzong Pill and Its Components on Central Cholinergic System in Mice with Learning and Memory Disorders Induced by Scopolamine

作　　者：褚松龄[1] 王学美[1] 富宏[1] 刘庚信[1] 

机构地区：[1]北京大学第一医院,北京100034

出　　处：《中国中医药信息杂志》2006年第2期18-19,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京大学985资助项目;首都医学发展科研基金(中医药重点项目;03-Ⅱ-04)

摘　　要：目的研究加味五子衍宗方及有效组分对东莨菪碱所致记忆获得障碍模型动物中枢胆碱能系统的影响。方法用氢溴酸东莨菪碱ip致小鼠记忆获得性障碍模型,Morris水迷宫实验之后,采用比色法检测小鼠脑乙酰胆碱含量及乙酰胆碱酯酶活性的变化。结果模型组小鼠脑乙酰胆碱含量、乙酰胆碱酯酶活性均显著低于空白对照组(P<0.01)。与模型组比较,加味五子衍宗复方组、总多糖组及总黄酮组小鼠脑乙酰胆碱含量显著升高(P<0.05,P<0.01),其中总多糖组明显超过复方组(P<0.05);总多糖组小鼠脑乙酰胆碱酯酶活性显著升高(P<0.05),其余各组有升高趋势,但无显著性差异(P>0.05)。结论加味五子衍宗方及其有效组分能够通过改变脑乙酰胆碱含量及乙酰胆碱酯酶活性提高中枢胆碱能系统功能,对东莨菪碱所引起的小鼠学习记忆障碍具有保护作用。Objective To investigate the effects of Jiawei Wuzi Yanzong Pill and its components on central cholinergic system in the model mice with learning and memory disorders induced by scopolamine. Methods Mice were treated by Jiawei Wuzi Yanzong Pill and its components for 15 days and induced by ip scopolamine. After the experiment in Morris water maze, the content of acetylcholine and the activity of acetylcholinesterase from brains of mice were determined by colorimetry. Results Significant differences were observed between model group and naked group that the content of acetylcholine and the activity of acetylcholinesterase from brains of model mice were decreased （P〈0.01）. Compared with model group, Jiawei Wuzi Yanzong Pill and its components treated groups increased the content of acetylcholine （P〈0.05, P〈0.01）, and the content of acetylcholine of flavonoids group was higher than the compound group of Jiawei Wuzi Yanzong Pill （P〈0.05）, the activity of acetylcholinesterase of flavonoids group were markedly increased compared with model group （P〈0.05） and that of other groups inclined to be increased but significant differences were not observed （P〉0.05）. Conclusion Jiawei Wuzi Yanzong Pill and its components could improve the function of central cholinergic system by increasing the content of acetylcholine and the activity of acetylcholinesterase and possesses a certain preventive effects on mice with learning and memory disorders induced by scopolamine.

关 键 词：五子衍宗丸 黄酮类 多糖类 东莨菪碱 学习障碍 记忆障碍 乙酰胆碱 乙酰胆碱酯酶 

分 类 号：R285.5[医药卫生—中药学]