电喷雾质谱法分析假单胞菌的代谢产物鼠李糖脂  

Identification of Rhamnolipids Produced by Pseudomonas sp.BS-03 with Electrospray Ionization-Mass Spectrometry

作　　者：沈薇[1] 李校堃[1] 杨树林[1] 宁长发[1] 

机构地区：[1]南京理工大学生物工程研究所,南京210094

出　　处：《分析化学》2006年第1期69-72,共4页Chinese Journal of Analytical Chemistry

基　　金：国家十五科技攻关项目(No.2001BA706B-18);南京市科技发展计划项目(No.200301045)资助课题

摘　　要：采用电喷雾质谱(ESI-MS)结合碰撞诱导解离(CA)技术,分析了假单胞菌BS-03,利用甘油产的鼠李糖脂提取物。根据一级和二级质谱图确定了提取物中存在23种鼠李糖脂组分,主要由4种物质(RhC10、RhC10C10、Rh2C10和Rh2C10C10)构成,其中前3种的丰度较高也较平均。该提取物中单鼠李糖脂的含量高于双鼠李糖脂,并且双鼠李糖脂的二级质谱图中普遍存在强度较高的m/z为205、247的特征碎片离子,而单鼠李糖脂中却不存在此特征碎片离子。Electrospray ionization-mass spectrometry （ESI-MS） and collisional activation technology were used to determine the constituents and their relative abundance of various rhamnolipids that were produced by Pseudomonas sp. BS-03 with glycol as sole carbon source. Twenty-three rhamnolipids were identified based on ESI-MS and MS2, the abundant components were RhC10, RhC10 C10, Rh2C10 and Rh2C10C10 in which the monorhamnolipids content was higher than that of dirhamnolipids. According to MS^2 there were characteistic fragment ions at m/z 205, 247 in all dirhamnolipids while it did not exist in any monorhamnolipids. It presumed m/z 205 was formed by fragment ion at m/z 309 （ dirhamnose radicle） collision with argon detached m/z 103. And m/z 247 came into being by fragment ion at m/z 309 （ dirhamnose radicle） first dehydrated between two rhamnose to form fragment ion at m/z 291 and then m/z 291 quickly decarboxyled. Accordingly the form mechanism of m/z 205,247 was deduced and were expressed as symbol of dirhamnolipid. the abundant fragment ions at m/z 205 and 247 in MS2

关 键 词：电喷雾质谱(ESI-MS) 碰撞诱导解离(CA) 鼠李糖脂 假单胞菌 

分 类 号：Q936[生物学—微生物学]