Hp培养滤液诱导下抑制hTERT基因对SGC7901细胞bcl-2蛋白表达的影响  

Effects of H.pylori incubating filtrate on expression of bcl2 protein in human 7901 gastric carcinoma cells transferred antisense hTERT

作　　者：王国安[1] 刘海峰[2] 房殿春[1] 腾小春[1] 何俊堂 陈刚[1] 

机构地区：[1]第三军医大学西南医院全军消化专科中心,重庆400038 [2]武警总医院消化内科,北京100039

出　　处：《西南国防医药》2006年第1期14-16,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：军队医药卫生"九五"重点课题资助项目NO.96Z047;重庆市应用基础研究基金资助项目;NO.1998-06

摘　　要：目的:探讨bcl-2表达与端粒酶活性二者之间的调控关系,了解Hp的致癌机制。方法:在Hp培养滤液诱导前后,采用流式细胞仪检测抑制hTERT基因之后SGC7901胃癌细胞bcl-2蛋白的表达。结果:对照组SGC7901胃癌细胞24 h、36 h和48 h表达bcl-2蛋白的阳性细胞率和荧光指数显著低于经Hp培养滤液诱导的SGC7901胃癌细胞组(P<0.05)。在Hp滤液诱导下,抑制hTERT基因的SGC791胃癌细胞24 h、36 h和48 h表达bcl-2蛋白的阳性细胞率和荧光指数与SGC7901胃癌细胞无显著差异(P>0.05)。结论:Hp感染可以促进bcl-2蛋白表达,这可能是Hp感染致胃癌的重要机制之一。端粒酶活化不参与调控bcl-2蛋白表达。Objective： To explore the relationship between the activity of telomerase and the expression of bcl - 2 protein and to study the carcinogenic mechanism of H. pylori. Methods： The expression of bcl - 2 protein in SGC7901 cells transferred antisense hTERT was detected by flow eytometryanalysator（FCM） in the presence of low concentration of H. pylori incubating filtrate. Results： The rate of cells and the fluorescent index of expressing bcl - 2 protein in the control SGC7901 cells were significantly lower than those in SGC7901 cells culture with H. pylori filtrate in 24 , 36 and 48 h（P〈0. 05）. It was not remarkable the differences of the rate of cells and fluorescent index of expressing bcl - 2 protein between SGC7901 cells and SGC7901 cells transferred anrisense hTERT both cultured with H, pylori filtrate in 24, 36 and 48 h（P〉0.05）. Conclusion： H. pylori infection could promote the expression of bcl - 2 protein in the SGC7901 cell, which may he an important cancerigenic mechanism of H. pylori. Telomerase does not take part in the regulation of the expression of bcl - 2 protein.

关 键 词：幽门螺杆菌 胃癌 端粒酶 端粒酶催化亚单位 bcl-2 

分 类 号：R286[医药卫生—中药学]