维甲酸对原发性高血压大鼠心肌血管平滑肌增殖的作用及其机制  

Effects of all-trans retinoic acid on myocardium and vascular smooth muscles in spontaneous hypertensive rats and its mechanism

作　　者：黄真[1] 张勤[1] 王国蓉[2] 黄俊[2] 

机构地区：[1]成都军区总医院药剂科,四川成都610083 [2]成都市第一人民医院,四川成都610021

出　　处：《西南国防医药》2006年第1期28-31,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的:探讨维甲酸对自发性高血压大鼠(SHR)心肌、血管平滑肌增殖的影响和肾素-血管紧张素-醛固酮系统(RAS)所起的作用。方法:18只SHR大鼠随机分为全反式维甲酸治疗组、对照组、卡托普利治疗组,每组6只。分别予治疗组和对照组皮下注射全反式维甲酸(ATRA)10mg/Kg体重或溶媒,高血压药物治疗组予卡托普利0.25mg/Kg体重管饲,每天一次,均疗程4周。疗程结束后观察各组SHR大鼠心肌、血管平滑肌(VSMC)增殖细胞核抗原(PCNA)的变化以及心肌ACE-mRNA、ACE2-mRNA的表达。结果:维甲酸治疗组心肌、血管平滑肌增殖细胞核抗原(PCNA)表达显著低于对照组(P<0.01),卡托普利治疗组的PCNA表达也显著低于对照组(P<0.01),但维甲酸治疗组与卡托普利组比较也有显著差异(P<0.01);维甲酸治疗组ACE-mRNA的表达低于卡托普利组及对照组(P<0.01),卡托普利组ACE-mRNA的表达也低于对照组(P<0.01);而维甲酸组ACE2-mRNA的表达则高于对照组及卡托普利组(P<0.01),后两组之间则无显著差异(P>0.05)。结论:维甲酸能抑制SHR模型心肌、血管平滑肌PCNA的表达,并在增加ACE2-mRNA表达的同时减少ACE-mRNA的表达,从而有可能干预增殖性血管疾病的病程。Objective. To discuss the effects of all - tram retinoic acid（atRA） on rennin - angiotensin system（RAS） and proliferation of myocardium and vascular smooth muscles in spontaneous hypertensive rats（SHR）. Methods： SHR were randomly divided into atRA treated group, dissolvent treated group and captopril treated group. Four weeks after treatment, the levels of ACE mRNA and ACE2 mRNA in myocardium were examined by transcription - polyraerase chain reaction.Expression of proliferating cell nudear antigen（PCNA） in vascular smooth muscle and myocardium were assessed by immunohistochemical staining. Results. Compared with the other two groups, the PCNA in myocardium and vascular＇smooth muscle decreased in atRA treated group（P〈0. 01） and the levels of ACE2 mRNA increased and ACE mRNA decreased markedly（P〈0. 01）. compared with the dissolvent treated group, the PCNA decreased also in eaptopril group（P〈0. 01）. onclusion. atRA treatment inhibits the expression of PCNA in myocardium and vascular smooth muscle and increases expression of ACE2 mRNA while decreases expression of ACE mRNA in myocardium, which resulting in the attenuation of myocardial damage in SHR Therefore, it is indicated that atRA may interfere with the proliferative vascular diseases.

关 键 词：维甲酸 原发性高血压 血管平滑肌增殖 ACE ACE2 

分 类 号：R543[医药卫生—心血管疾病]