兔血浆中盐酸阿扑吗啡的HPLC-荧光法测定  

Determination of Apomorphine Hydrochloride in Rabbits Plasma by HPLC with Fluorescence Detection

作　　者：江文明[1] 蒋新国[1] 陈钧[1] 高小玲[1] 陆伟[1] 

机构地区：[1]复旦大学药学院药剂学教研室,上海200032

出　　处：《中国医药工业杂志》2006年第2期113-115,共3页Chinese Journal of Pharmaceuticals

基　　金：国家自然科学基金(30171112)

摘　　要：建立了HPLC-荧光法测定兔血浆中盐酸阿扑吗啡的浓度。以吡哌酸为内标,采用C18柱,流动相为1mmol/LEDTA-2Na缓冲液(含0.1%三乙胺,磷酸调至pH3.2)-乙腈(85∶15)。荧光检测的激发波长270nm、发射波长450nm。血浆样品经液液萃取后进样测定。盐酸阿扑吗啡在4～500ng/ml范围内线性良好,定量限为4ng/ml。平均提取回收率均大于70%,日内、日间RSD均小于10%。A HPLC method with fluorescence detection was established for the determination of apomorphine hydrochloride in rabbits plasma extracted with CH2Cl2. Pipemidic acid was used as the internal standard. A C18 column was used with the mobile phase of 1 mmol/L EDTA-2Na buffer solution （containing 0.1% triethylamine, adjusted pH to 3.2 by phosphoric acid）-acetonitrile （85 ： 15）. The excitation wavelength and emission wavelength were 270 and 450nm, respectively. The calibration curve was linear in the range of 4 - 500 ng/ml with the quantitation limit of 4 ng/ml. The average extraction recovery was more than 70%. The RSDs of intra- and inter-day were less than 10%.

关 键 词：阿扑吗啡 血药浓度 药物动力学 高效液相色谱 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]