竹黄无性型菌株产竹红菌素的研究  

Hypocrellins produced by anamorphosis fermentation of Shiraia bambusicola

作　　者：陈佳佳[1] 李兆兰[1] 焦庆才[1] 

机构地区：[1]南京大学生命科学学院药物生物技术国家重点实验室,江苏南京210093

出　　处：《中草药》2006年第1期48-50,共3页Chinese Traditional and Herbal Drugs

基　　金：国家技术创新基金资助项目(02CJ-13-01-16)

摘　　要：目的获得能稳定产生竹红菌素的竹黄无性型菌株,通过人工培育获得其活性成分竹红菌甲素。方法通过组织分离,从野生竹黄子座中分离筛选出无性型菌株0258,进行人工条件下的发酵培养。对其发酵产物进行提取、分离和纯化,通过理化性质测定和波谱(UV/V IS、IR、M S)技术进行结构鉴定。结果从竹黄子实体分离筛选到一株无性型菌株0258,其能产生稳定的竹红菌甲素。结论首次获得稳定产生竹红菌素的竹黄无性型菌株,解决了竹黄人工培育的种源问题,使竹红菌素的工业化生产成为可能。Objective To obtain anamorphosis of Shiraia bambusicola producing the component, hypocrellin A effectively. Methods A strain was isolated from the fruit of S. bambusicola by separating its hypha and was cultured by solid state fermentation. One of the pure chemical compounds isolated from the stroma of wild S. bambusicola was studied on the basis of UV/VIS, IR, MS spectrometric analysis and physicochemical constants. Results The pigment is proved to be hypocrellin A. The isolate was determined as the stable anamorphosis strain 0258 of S. bambusicola. Conclusion A namorphosis strain of S. bambusicola is obtained and precisely appraised for the first time. This study will provide basis for the future massively industrialized hypocrellin production.

关 键 词：竹黄 竹红菌甲素 无性型 波谱分析 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]