河南栾川地区“白化”丹参的TLC和HPLC图谱分析  

Quality analysis of 'whitish' Radix Salvia Miltiorrhiza in Luanchuan Region of Henan Province by TLC and HPLC

作　　者：赵瑞娜[1] 谢培山[1] 尹卫平[1] 卢平华 颜玉贞 王忠东[1] 

机构地区：[1]河南科技大学化工与制药学院,河南洛阳471003 [2]珠海科曼中药研究有限公司,广东珠海519085

出　　处：《中草药》2006年第1期119-122,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的借助丹参药材TLC和HPLC图谱的条件,对河南栾川地区“白化”丹参(因生长环境影响而根部表皮及断面呈白色)与正常丹参的品质进行色谱图谱分析,比较成分的异同。方法TLC色谱条件:GF254预制薄层板,水溶性部位与脂溶性部位分别展开、显色;HPLC色谱条件:Zorbax SB C18色谱柱,水溶性部位与脂溶性部位分别进行梯度洗脱。结果栾川地区“白化”丹参脂溶性有效成分明显偏低,仅检出痕量的二萜醌类成分;水溶性部分丹参酚酸类成分较高。结论栾川地区丹参根的“白化”现象反映了生长环境可影响丹参二萜醌类成分的生物合成,具体影响因素待进一步考察,是值得丹参GAP管理注意的问题。Objective To study the sample quality of‘whitish＇ Radix Salvia Miltiorrhiza （the root of a grown environmental-influenced variant sample of Salvia miltiorrhiza, which cortex and transection is white） grown in Luanchuan Region of Henan Province and normal Radix Salvia Miltiorrhiza by the means of TLC and HPLC chromatographic fingerprints;hence to compare the difference of their constituents. Methods TLC Chromatographic condition： Precoated silica gel F254 plate; hydrophilic and lipophilic constituents were developed and derivatizated with different solvent systems and visualization reagents, respectively. HPLC Chromatographic condition： Zorbax SB C18 column;hydrophilic and lipophilic constituents were gradient eluted, respectively. Results The ‘whitish＇ Radix Salvia Miltiorrhiza contains lower content of lipophilic compounds, the main diterpene quinones was only trace amount, while the content of hydrophilic constituents was relatively higher. Conclusion The ‘whitish＇ phenomenon suggests that the ‘micro＇ environmental condition can probably influence negatively the bio synthesis of diterpenequinones. The factors of influence still need further study. Such phenomenon would be worth concerning seriously for GAP administration.

关 键 词：丹参 “白化”丹参 薄层色谱 高效液相色谱 

分 类 号：R282.710.3[医药卫生—中药学]