噬菌体展示技术在鼠CD137L活性研究中的应用  

Application of phage display techinique in study of rat CD137L activity

作　　者：夏西燕[1] 张利宁[1] 庄学伟[2] 王妍[2] 高锐[3] 刘军莉[4] 张蘋[1] 高飞[1] 

机构地区：[1]山东大学医学院,山东济南250012 [2]山东大学齐鲁医院 [3]山东省济南卫生学校 [4]山东大学第二医院

出　　处：《山东医药》2006年第2期4-6,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30371352)

摘　　要：目的探讨噬菌体展示技术在鼠CD 137配体(CD 137L)活性研究中的应用。方法用PCR法扩增出鼠CD 137L胞外区,用噬菌体展示技术展示其CD 137L;EL ISA方法检测噬菌体CD 137L的抗原性,并分3次注射到大鼠体内,取血清检测大鼠体内多克隆抗体水平。结果PCR方法成功扩增出鼠CD 137L胞外区;获得重组子pCom b3H-CD 137L,成功展示在噬菌体上;噬菌体CD 137L可与抗CD 137L特异性结合;大鼠体内产生了CD 137L的多克隆抗体。结论噬菌体展示技术用于鼠CD 137L活性的研究,可提高制备特异性抗体的成功率,得到特定结构和功能的蛋白质。Objective：To explore the application of phage display technique in study of rat CD137L activity. Methods; Amplify the extracellular region of mouse CD137L by PCR method, dispay it through phage display technique, and detect its antigenicity by ELISA. CD137L was injected into rats by three times and the level of polyclonal antibody in the rats sera was detected. Results; The extracellular region of mouse CD137L was successfully amplified. The reeon pComb3H-CD137L was obtained and successfully displayed in the phage. The phage CD137L could specifically bind with anti-CD137L. CD137L polyclonal antibody was found in the rats. Conclusion： Phage display technique in the study of mouse CD137L activity can elevate the achievement radio of preparation for polyelonal antibody and can obtain proteins with definite structure and function.

关 键 词：细菌噬菌体 载体蛋白 展示技术 

分 类 号：R392.1[医药卫生—免疫学]