紫外线照射充氧自体血心脏停搏液对体外循环中心肌保护作用的研究  

Myocardial protective effect of UBIO as cardiac arrestingsolution in the process of CPB

作　　者：向道康[1] 柏隆书[1] 涂丽萍[1] 蔡鸿[1] 

机构地区：[1]贵州省人民医院,贵州贵阳550002

出　　处：《山东医药》2006年第2期7-9,共3页Shandong Medical Journal

基　　金：贵州省自然科学基金课题(黔科通[2003]50号)

摘　　要：目的研究紫外线照射充氧自体血(U B IO)心脏停搏液冠状动脉间断顺行灌注对体外循环(CPB)中心肌的保护作用。方法将20只健康成年杂种犬(雌雄不拘)随机分为实验组和对照组,每组10只。CPB中实验组采用紫外线照射充氧自体血心脏停搏液间断灌注;对照组用等量4℃S t.T hom asⅡ号冷晶体液作为心脏停搏液间断灌注,两组其余操作相同。结果开放升主动脉后,实验组冠状静脉窦血清cT n I、CK-M B水平和左心室心肌M DA水平低于对照组(P<0.05),左心室心肌SOD活性和ATP水平高于对照组(P<0.05);心脏自动复跳率明显高于对照组(P<0.01)。心肌超微结构观察显示,实验组左心室心肌纤维结构完整,细胞内线粒体轻度水肿,而对照组心肌纤维部分断裂、溶解,线粒体基质外溢,糖原颗粒减少。结论U B IO血心脏停搏液有较好的心肌保护作用,且能减少心脏停搏液的灌注次数,延长心脏停搏时间;本研究为U B IO血心脏停搏液的临床应用奠定了基础。Objectives To investigate the myocardial protective effect of Ultraviolet Autoblood Irradiation and Oxygenation （UBIO） as cardiac arresting solution in the process of cardiopulmonary bypass （CPB） in canine, Methods：20 healthy mongrel dogs （15-20kg） were randomly divided into two groups. The experimental group was infused with UBIO blood as an cardiac arresting solution via the ascending aorta, while the control group was given 4℃C St. Thomas II cold crystal liquid. Results： The levels of cTnI, CK-MB in coronary venous sinus serum and MDA in myocardial tissue in experimental group were significantly lower than that in the control group （P〈0. 05）,but the contents of ATP and SOD in myocardial tissue was significantly higher （P〈（0. 05）. The postoperative cardiac auto-reheating rate in the experimental group was significantly higher than that in the control group （P〈0.01）. Histological examination showed that the mitochondria in the cardial cell was a little slight swelling and the structure of the myocardial fiber was almost normal in the experimental group. While in the control group part of myocardial fiber could be observed broken, some dissolved, with pouring out of the contents and disappearance of glycogen particles. Conclusion： UBIO blood used for cardiac arresting solution in the process of CPB can possess a better protective effect on myocardium as well as decrease the perfusion times and prolong the cardiac arresting time. This study demonstrates the possibility of furtherclinical application of UBIO surgery with CPB.

关 键 词：心肌保护 自体血 充氧 体外循环 

分 类 号：R454.2[医药卫生—治疗学]