化学诱导剂对血小板钙离子浓度变化的影响  

Ca ^(2+)Concentration change in platelets activated by chemical agonist

作　　者：邓新立[1] 丛玉隆[1] 李绵洋[1] 王俊玫[1] 

机构地区：[1]解放军总医院临床检验科,北京100853

出　　处：《中华检验医学杂志》2006年第1期55-57,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的探讨化学诱导剂作用下不同人群血小板钙离子浓度的变化。方法(1)以不同浓度的ADP、凝血酶、胶原、花生四烯酸、5HT激活血小板,用流式细胞仪观察20例正常健康人血小板钙离子浓度变化。(2)用ADP、胶原、花生四烯酸、5HT激活血小板,用流式细胞仪观察心肌梗死患者及高血压患者(各20例)血小板钙离子浓度的变化。(3)用花生四烯酸激活血小板,用流式细胞仪观察20例心肌梗死患者口服阿司匹林前后血小板钙离子浓度的变化。结果(1)ADP、瑞斯托酶素、胶原、花生四烯酸、5HT血小板激活剂均可导致血小板钙离子浓度升高(P<0.05),而且在一定范围内,钙离子浓度的升高幅度随激活剂的浓度升高而增加。没有观察到凝血酶导致血小板钙离子浓度升高。(2)同样激活剂刺激下,心肌梗死患者和高血压患者血小板钙离子浓度升高的幅度均高于正常健康人(P<0.05)。(3)服用阿司匹林后心肌梗死患者血小板钙离子浓度下降的幅度低于正常健康人(P<0.05)。结论化学诱导剂对血小板的激活过程与钙离子浓度的变化密切相关;心肌梗死、高血压患者可能存在血小板钙通道活性增高;钙离子能反应服用阿司匹林后血小板的功能状态,检测血小板钙离子对阿司匹林的疗效监测有一定的价值。Objective To observe the Ca^2＋ concentration changes in the platelets activated by chemical agonist in different study group treated with aspirin. Methods We measured the Ca^2＋ concentration in the platelets activated by different concentration of ADP, thrombin, collagen, arachidonic acid and 5-HT with the flowcytometer in normal health （n = 20）. We measured the Ca^2＋ concentration in the platelets activated by ADP, collagen, arachidonic acid and 5-HT in myocardial infarction （n = 20） and hypertension （n =20）. We measured the Ca^2＋ concentration in the platelets activated by arachidonic acid in myocardial infarction （n = 20 ） treated with aspirin. Results As the concentrations of ADP, thrombin, collagen, arachidonic acid and 5-HT increased, the Ca^2＋ concentration in the platelets increased. There was the relation between agonist concentration and Ca^2＋ concentration. Activated by the same agonist, the Ca^2＋ concentration in the platelets showed more increase in myocardial infarction and hypertension than in normal health. The Ca^2＋ concentration show less increase in myocardial infarction treated with aspirin than in normal health. Conclusion The procedure which chemical agonist activating platelets is related to Ca^2＋ concentration. The activity of Ca^2＋ channels in platelets is high in myocardial infarction and hypertension. Examining the Ca^2＋ concentration in platelets is helpful to supervise the aspirin therapy in myocardial infarction.

关 键 词：血小板 钙离子 血小板钙离子浓度 浓度变化 诱导剂 化学 血小板激活剂 心肌梗死患者 花生四烯酸 流式细胞仪 

分 类 号：R446.1[医药卫生—诊断学]