东海原甲藻的分子鉴定  

Molecular identification on Prorocentrum donghaiense

作　　者：罗立明[1] 胡鸿钧[1] 李夜光[1] 齐雨藻[2] 吕颂辉[2] 耿亚红[1] 邓光[1] 

机构地区：[1]中国科学院武汉植物园/武汉植物研究所,湖北武汉430074 [2]暨南大学水生生物研究所,广东广州520632

出　　处：《海洋学报》2006年第1期127-131,共5页

基　　金：国家重点基础研究发展规划资助项目(2001CB409701)

摘　　要：采用nrDNA ITS序列及18S序列两种分子标记,对东海原甲藻和美国国家海洋藻种保藏中心(CCMP)的具齿原甲藻进行分子鉴定,结果表明,两者之间序列非常相似,两者的nrDNA ITS序列碱基差异值仅为0.002,nrDNA 18S序列的碱基差异值为0.000,根据分子数据,东海原甲藻和美国国家海洋藻种保藏中心的具齿原甲藻应为同一个种.从基因库获取原甲藻的另外3个种nrD-NA ITS序列和8个种的18S序列,用计算机软件进行分析和构建分子系统树,结果显示18S序列用于原甲藻的分子鉴定过于保守,而nrDNA ITS更适合于该种属界定的分子标准.Prorocentrum donghaiense and P. dentatum from CCMP were re-identified by two molecular makers-nrDNA ITS and 18S sequences. The sequences of these two species are very similar. The results show that the nrDNA ITS nucleotide divergence between P. donghaiense and P. dentatum CCMP is only 0. 002 and 18S nucleotide divergence is 0. 000. According to the molecular data, P. donghaiense and P. dentatum CCMP are a similar species. Other three nrDNA ITS sequences and eight 188 sequences of Prorocentrum were obtained from the Genbank. These sequences were analyzed and the phylogenetic trees were constructed by computer programme （Clustal X and Mega）. The results show that an nrDNA ITS region is a more suitable molecular maker to identifying interspecies of Prorocentrum and 18S sequence is too conservative.

关 键 词：东海原甲藻 具齿原甲藻 NRDNA ITS NRDNA 18S 分子鉴定 

分 类 号：Q948.885.3[生物学—植物学]