川芎嗪对肝缺血再灌注损伤大鼠P-选择素表达的影响  

Effects of ligustrazine on P-selectin expression of rat liver with ischemia-reperfusion injury and construction of liver cell

作　　者：何文智[1] 熊艾君[1] 陈美丽[1] 陈北阳[1] 陈安[1] 

机构地区：[1]湖南中医学院解剖学和组胚学教研室,湖南长沙410007

出　　处：《中国中西医结合消化杂志》2006年第1期1-5,共5页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：湖南省科技厅资助项目(B0082)

摘　　要：[目的]观察川芎嗪干预肝缺血再灌注损伤大鼠P-选择素表达,探索可能的病理机制,为预防治疗肝细胞缺血再灌注损伤提供依据。[方法]将64只SD大鼠随机分为4组,肉眼观察肝脏形态变化,光镜、电镜观察肝细胞形态变化,免疫组化SABC法检测肝组织中P-选择素表达。[结果]缺血再灌注组可见肝细胞水肿明显,伴空泡形成,间质充血水肿及炎性细胞浸润,再灌注6 h可见肝组织出现明显的点状坏死灶,并可见到灶性坏死;再灌注24 h肝组织可见多发性灶性坏死及片状坏死灶。川芎嗪干预组各时间点肝组织外观与正常肝相似,光镜下肝细胞无明显肿胀,有极少量空泡形成,但无变性或坏死,且间质无明显变化。免疫组化SABC法检测显示,缺血再灌注早期P-选择素即在肝组织中表达,与川芎嗪干预组及假手术组比较,差异有统计学意义(P<0.01)。缺血再灌注组P-选择素表达以1 h时最为明显,与再灌6 h及24 h相比,差异有统计学意义(P<0.01)。[结论]川芎嗪能有效抑制P-选择素的表达,减轻肝缺血再灌注损伤。P-选择素主要在缺血再灌注损伤早期表达。P-选择素介导中性粒细胞滚动黏附,可能是肝缺血再灌注损伤的主要机制之一。[Objective] To observe the pathologic changes of liver cell in ischemia-reperfusion injury rat under the microscope and electron microscope, and explore the pathologic mechanism of liver ischemia-reperfusion and the effects of ligustrazine. [Methods] Sixty^four Sprague-Danley （SD） rats were devided into four groups. Morphology of liver, hepatic tissue and expression of P- selectin in various groups were observed. [Results] In the hapetic ischemia-reperfusion group, dropsy of liver tissue was obvious and vacuole came into being. Histological damage in point was found after reperfusion 6 h and more serious after reperfusion 24 h. These changes were markedly alleviated in the group of ligustrazine treatmemt. P-selectin expressed in early ischemia-reperfusion injury period, which had significant difference with ligustrazine treated group and pseudooperation group （P〈S0.01）, and expressed much obviously at ischemia-reperfusion 60 min, which was more obviously than that at 6 h and 24 h （P〈0. 01）. [Conclusion]P-selectin expressed in early ischemia-reperfusion injury period. Ligustrazine could restrict the expression of P-selectin and reduce the hepatic injury caused by the ischemia-reperfusion. P-selectin mediated neutrophil rolling and adherence, which might contribute to liver ischemia reperfusion injury.

关 键 词：肝脏 再灌注损伤 P-选择索 川芎嗪 微循环 

分 类 号：R657.3[医药卫生—外科学] R619[医药卫生—临床医学]